Skip to main content
ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Infectious Bacterial Diseases Research » Research » Publications at this Location » Publication #188313
Title: DIFFERENTIAL IN VIVO EFFECTS OF WHOLE CIGARETTE SMOKE EXPOSURE VERSUS CIGARETTE SMOKE EXTRACT ON MOUSE CILIATED TRACHEAL EPITHELIUM

Author
item Elliott, Margaret
item SISSON, JOSEPH - UNIV. OF NE MEDICAL CNTR
item WEST, WILLIAM - UNIV. OF NE MEDICAL CNTR
item WYATT, TODD - UNIV. OF NE MEDICAL CNTR

Submitted to: Experimental Lung Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/1/2006
Publication Date: 3/1/2006
Citation: Elliott, M., Sisson, J.H., Wyatt, T.A. 2006. Differential in vivo effects of whole cigarette smoke exposure versus cigarette smoke extract on mouse ciliated tracheal epithelium. Experimental Lung Research. 32(3):99-118.

Interpretive Summary: Currently there are a number of different animal models of cigarette smoke exposure. One such method allows mice to inhale cigarette smoke extract (a liquid made from cigarette smoke) (CSE) through the nose. This method can cause inflammation in the lungs with a short exposure. We wanted to determine if the affect of CSE inhalation on the upper airway was the same as that seen with inhalation of smoke from burning cigarettes. The results indicated that the two treatments were not the same. We found that CSE causes a greater inflammatory response in the lung, alters enzyme activity in cells in the upper airway and alters the beating of cilia in the trachea in a manner different from inhalation of regular cigarette smoke given for the same amount of time. These results indicate that the two treatments are not the same and this should be taken into consideration when choosing a model of exposure.

Technical Abstract: In this study we compared the affect of vapor phase cigarette smoke (CS) versus cigarette smoke extract (CSE) on the lungs and upper airway of C57BL/6 mice. We found that CSE treatment significantly increased neutrophil influx (P<0.001), baseline ciliary beat frequency (CBF) (P<0.05), and protein kinase C activity compared to CS and controls. Isoproterenol increased CBF with CS exposure, but decreased CBF with CSE ( P<0.01). Isoproterenol increased PKA activity in all groups except CSE. CSE exposure induced inflammatory cell bronchiolitis. These data indicate that CSE exposure has differential effects on the lungs and tracheal epithelium compared to CS exposure.