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Title: PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES PRODUCED AGAINST AVIAN METAPNEUMOVIRUS SUBTYPE C WHICH REACT AGAINST THE NUCLEOCAPSID PROTEIN

Author
item Yu, Qingzhong
item Estevez, Carlos
item Kapczynski, Darrell

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: 1/17/2006
Publication Date: 7/18/2006
Citation: Yu, Q., Estevez, C., Kapczynski, D.R. 2006. Production and characterization of monoclonal antibodies produced against Avian Metapneumovirus Subtype C which react against the nucleocapsid protein [abstract]. In: Proceedings of the 2006 Annual Meeting of American Association of Avian Pathologists, July 16-19, 2006, Honolulu, HI. p. 164.

Interpretive Summary:

Technical Abstract: Monoclonal antibodies (MAbs) were prepared against avian metapneumovirus (aMPV) subtype C virus (aMPV/Minnesota/turkey/1a/97). Six MAbs were selected based on ELISA activities and characterized by isotyping, neutralization test, Western blot analysis, and immunohistochemistry assay. The results showed all six MAbs reacted with the nucleocapsid (N) protein of aMPV, but did not neutralize aMPV infectivity at a detectable level. Three MAbs (3E, 9D and 12C) belonged to IgG1 subclass, whereas the other three (5D, 8E and 16E) were related to the IgG2a subclass. These MAbs provide new tools and methods for investigating aMPV infection, pathogenesis, and diagnosis of aMPV disease.