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ARS Home » Research » Publications at this Location » Publication #189382
Title: A POD NECROSIS DISEASE (CHOCOLATE POD) OF SNAP BEAN (PHASEOLUS VULGARIS) IN OREGON CAUSED BY A STRAIN OF CLOVER YELLOW VEIN VIRUS

Author
item Larsen, Richard
item MYERS, J - OSU, CORVALLIS

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 3/30/2005
Publication Date: 6/1/2005
Citation: Larsen, R.C., Myers, J.M. 2005. A pod necrosis disease (chocolate pod) of snap bean (phaseolus vulgaris) in oregon caused by a strain of clover yellow vein virus. Phytopathology. American Phytopathology Society online http://www.apsnet.org/meetings/div/pc05abs.asp.

Interpretive Summary:

Technical Abstract: Clover yellow vein virus (ClYVV) typically causes severe leaf mosaic symptoms and stunting in susceptible snap beans. During 2004, field-grown plants in the Willamette Valley, Oregon were observed with top necrosis and light brown discoloration on pods or “chocolate pod.” Leaf and pod samples were collected and analyzed by ELISA using a potyvirus group-specific monoclonal antibody (MaB) (Agdia, Inc.) and a polyclonal antiserum to Bean yellow mosaic virus (BYMV). Total nucleic acid was also extracted and analyzed by RT-PCR using primers specific for BYMV and ClYVV. Positive results were obtained with the potyvirus MaB but not with antiserum to BYMV. Amplicons of the expected size were generated in RT-PCR reactions using primers specific only for ClYVV. The virus isolate characteristics were similar to a strain of ClYVV detected in the Great Lakes region in 2000-2004 transmitted by the soybean aphid (Aphis glycines), except that pod necrosis was more severe in that region and the virus was not detectable by ELISA using the potyvirus MaB.