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ARS Home » Pacific West Area » Pullman, Washington » Grain Legume Genetics Physiology Research » Research » Publications at this Location » Publication #189483
Title: GENETIC TRANSFORMATION OF SCLEROTINIA SCLEROTIORUM THROUGH AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION.

Author
item WAMATU, JOHN - WASHINGTON STATE UNIV
item White, David
item Chen, Weidong
item WANG, XIN - WASHINGTON STATE UNIV

Submitted to: North American Pulse Improvement Association
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/2005
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: In order to study genetic factors of pathogenicity, insertional mutants of Sclerotinia sclerotiorum were generated using Agrobacterium tumefacience-mediated transformation with both mycelial fragments and ascospores. Transformants were tested for number of insertions by Southern hybridization and for stability of hygromycin B resistance, and are being screened for altered pathogenicity on lentil plants and for production of oxalic acid. The tagged mutations will allow identification of mutated genes.