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Title: Report of the Committee on Bluetongue and Bovine Retroviruses

Author
item PEARSON, JAMES - APHIS-RETIRED
item Wilson, William - Bill

Submitted to: United States Animal Health Association Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 7/17/2006
Publication Date: 10/17/2006
Citation: Pearson, J.O., Wilson, W.C. 2006. Report of the Committee on Bluetongue and Bovine Retroviruses-2005. United States Animal Health Association Proceedings. 110:161-169. 2006.

Interpretive Summary: Molecular biology of bluetongue and related viruses has provided the foundation for the development early warning technologies for indigenous and exotic disease outbreaks. The phylogenetic analysis of two-conserved target genes, one that is highly expressed in infected mammalian cells the other highly expressed in infected insect cells, from BTV prototype strains indicated that a complex primer design will be necessary for a comprehensive gene amplification diagnostic test. Status of the application of real-time RT/PCR and other existing and developing technologies for early warning of an exotic BTV outbreak was discussed.

Technical Abstract: Molecular biology of bluetongue and related viruses has provided the foundation for the development early warning technologies for indigenous and exotic disease outbreaks. The phylogenetic analysis of two-conserved target genes, one that is highly expressed in infected mammalian cells the other highly expressed in infected insect cells, from BTV prototype strains indicated that a complex primer design will be necessary for a comprehensive gene amplification diagnostic test. Status of the application of real-time RT/PCR and other existing and developing technologies for early warning of an exotic BTV outbreak was discussed.