Author
ARIAS, COVA - AUBURN UNIVERSITY | |
OLIVARES-FUSTER, OSCAR - AUBURN UNIVERSITY | |
HAYDEN, KARL - AUBURN UNIVERSITY | |
Shoemaker, Craig | |
GRIZZLE, JOHN - AUBURN UNIVERSITY | |
Klesius, Phillip |
Submitted to: Journal of Aquatic Animal Health
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 9/5/2006 Publication Date: 3/29/2007 Citation: Arias, C.R., Olivares-Fuster, O., Hayden, K., Shoemaker, C.A., Grizzle, J.M., Klesius, P.H. 2007. First report of Yersinia ruckeri biotype 2 in the USA. Journal of Aquatic Animal Health 19:35-40. Interpretive Summary: An extensive characterization of atypical Yersinia ruckeri isolates from hatchery reared brown trout from South Carolina was performed in this study. Yersinia ruckeri isolates were biochemically and genetically distinct from reference cultures but were unequivocally ascribed to Y. ruckeri species based on biochemical tests including semi-automatic identification systems (API 20E, VITEK), fatty acid methyl ester profiles (FAME) and 16S rRNA gene sequencing analysis. These trout isolates were non-motile and unable to hydrolyze Tween 20/80 and therefore were classified as Y. ruckeri biogroup 2. Genetic fingerprinting of the isolates showed biogroup 2 as a homogeneous group distinguishable from other Y. ruckeri isolates by genetic fingerprinting. This is the first report of Y. ruckeri biogroup 2 in the USA. Technical Abstract: A polyphasic characterization of atypical Yersinia ruckeri isolates from hatchery reared brown trout from South Carolina was performed in this study. Yersinia ruckeri isolates were biochemically and genetically distinct from reference cultures but were unequivocally ascribed to Y. ruckeri species based on API 20E, VITEK, FAME, and 16S rRNA gene sequencing analysis. These trout isolates were non-motile and unable to hydrolyze Tween 20/80 and therefore were classified as Y. ruckeri biogroup 2. Genetic fingerprinting of the isolates showed biogroup 2 as a homogeneous group distinguishable from other Y. ruckeri isolates by ERIC-PCR and AFLP typing. These is the first report of Y. ruckeri biogroup 2 in the USA. |