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Title: EST-SSR MARKERS FROM FRAGARIA VESCA L. CV. YELLOW WONDER

Author
item Bassil, Nahla
item NJUGUNA, WAMBUI - OREGON STATE UNIV
item Slovin, Janet

Submitted to: Molecular Ecology Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/6/2006
Publication Date: 5/2/2006
Citation: Bassil, N.V., Njuguna, W., Slovin, J.P. 2006. Est-ssr markers from fragaria vesca l. cv. yellow wonder. Molecular Ecology Notes. 6: 906-809

Interpretive Summary: This research addresses the problem of why high temperatures affect the growth and quality of strawberry fruit, making it impossible for growers to expand their production season into the hot summer months. Gene sequences were obtained, and these sequences were examined for repeating patterns that would allow us to identify them using a simple test. It is now easier to see if that gene, or a gene linked to it, is present when breeding strawberries that are more resistant to higher temperatures. These tests were used on a large number of different species of strawberry and gave information on how closely these different types strawberry are related to each other. Scientists who want to study genes that help plants cope with high temperatures, and breeders who want to develop new varieties of strawberries more quickly will benefit from this work.

Technical Abstract: Fourteen microsatellite primer pairs were developed from a cDNA library of Fragaria vesca cv. Yellow Wonder. Transferability to 13 species of Fragaria ranged from 71% in diploid species F. gracilis Losinsk., F. iinumae Makino, F. nilgerrensis Schltdl. ex J. Gay and F. nipponica Makino to 100% in the octoploid strawberry and its progenitors F. chiloensis (L.) Duch. and F. virginiana Duch. Polymorphism was high in polyploid Fragaria species, including F. orientalis, F. moshata, and the octoploid species. However, polymorphism and heterozygosity in 14 F. vesca genotypes using 8 of these EST-SSRs was low.