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Title: Analysisi of Tall Fescue Ests Representing Abiotic Stresses, Tissue Types and Developmental Stages

Author
item Mian, Rouf
item ZHANG, YAN - FORAGE IMP DIV, OK
item WANG, ZENG-YU - FORAGE IMP DIV, OK
item ZHANG, JIYI - FORAGE IMP DIV, OK
item CHENG, XIOFEI - FORAGE IMP DIV, OK
item CHEN, LEI - FORAGE IMP DIV, OK
item MAO, CHUNHONG - VIRGINIA TECH, VA
item DAI, XIBIN - PLANT BIO DIV, OK
item HE, JI - PLANT BIO DIV, OK
item ZHAO, XUECHUN - PLANT BIO DIV, OK
item SCOTT, ANGELA - PLANT BIO DIV, OK
item MAY, GREGORY - PLANT BIO DIV, OK

Submitted to: BMC Plant Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/4/2008
Publication Date: 3/4/2008
Citation: Mian, R.M., Zhang, Y., Wang, Z., Zhang, J., Cheng, X., Chen, L., Mao, C., Dai, X., He, J., Zhao, X., Scott, A.D., May, G.D. 2008. Analysisi of Tall Fescue Ests Representing Abiotic Stresses, Tissue Types and Developmental Stages. Biomed Central (BMC) Plant Biology. 8:22.

Interpretive Summary: Expressed sequenced tags (ESTs) typically represent the transcribed portion of a genome and they are an excellent resource for genomics studies. Although tall fescue has high agricultural importance, no fescue ESTs data were publicly available. In this paper we report the release of 44,516 high-quality tall fescue ESTs generated from nine cDNA libraries representing tissues from different plant organs, developmental stages, and abiotic stress factors. These ESTs were made publicly available through the NCBI dbEST (http://www.ncbi.nlm.nih.gov/dbEST) in September 2005. Later a Festuca Gene Index (FaGI) was established by TIGR (http://www.tigr.org/tigr-scripts/tgi/T_release.cgi?species=F_arundinacea) for annotation of these ESTs. These ESTs are an excellent source for development of PCR based molecular markers such as sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs). This large-scale ESTs database provides a very useful resource for genomics studies of tall fescue and other closely related forage and turfgrass species, including ryegrasses (Lolium sp.), meadow fescue (F. pratensis) and tetraploid fescue (F. arundinacea var glaucescens).

Technical Abstract: Tall fescue (Festuca arundinacea Schreb) is a major cool season forage and turf grass species in the temperate regions of the world. A total of 44,516 high-quality expressed sequence tags (ESTs) were generated from nine cDNA libraries of tall fescue representing tissues from different plant organs, developmental stages, and abiotic stress factors. These ESTs were made publicly available through the NCBI dbEST (http://www.ncbi.nlm.nih.gov/dbEST) in September 2005. Later a Festuca Gene Index (FaGI) was established by TIGR (http://www.tigr.org/tigr-scripts/tgi/T_release.cgi?species=F_arundinacea) for annotation of these ESTs. Currently, these are the only tall fescue ESTs available in the public sector. This large-scale ESTs database provides a very useful resource for genomics studies of tall fescue and other closely related forage and turfgrass species, including ryegrasses (Lolium sp.), meadow fescue (F. pratensis) and tetraploid fescue (F. arundinacea var glaucescens). This database will be highly useful for comparative genomics of tall fescue with other major grass species, including rice and wheat. These ESTs are an excellent source for development of PCR based molecular markers, including simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs).