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Title: IDENTIFICATION AND FUNCTIONAL ANNOTATION OF UNIQUE EST SEQUENCES BETWEEN VITIS SHUTTLEWORTHII AND V. VINIFERA GRAPES

Author
item LU, J - FLORIDA A&M UNIVERSITY
item HONG, H - FLORIDA A&M UNIVERSITY
item Hunter, Wayne

Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/5/2006
Publication Date: 1/15/2006
Citation: Lu, J., Hong, H., Hunter, W.B. 2006. Identification and functional annotation of unique est sequences between vitis shuttleworthii and v. vinifera grapes. Proceedings of International Plant and Animal Genome IX Conference. P. 18. p. 106.

Interpretive Summary:

Technical Abstract: A set of 2,140 gene sequences were isolated, identified, and determined to be unique to the grape Vitis shuttleworthii. Vitis shuttleworthii is a North American grape variety that is one of the most disease and pest resistant grape species originating in the Southeastern United States. From this unique set of sequences 200 have been used to design genetic markers for use in the current grape breeding program at Florida A & M University. These are used to aid selection of each years crosses, and have so far identified ~400 grape seedlings with increased disease, and insect resistance. In addition to the approximate 190,000 ESTs from about 70 different cDNA libraries of the European grapes (Vitis vinifera), ESTs from several North American grape species, including V. shuttleworthii, V. aestivalis, V. raparia, and V. rotundifolia have been generated during last few years. The largest EST data set among the North American grapes was generated from V. shuttleworthii, by Lu & Hunter in 2004, who sequenced 18,000 ESTs from a single cDNA library derived from mixed tissues of V. shuttleworthii. The discovery rate of new transcripts at 18,000 EST’s produced was 20%. A total of 8,500 unigenes were generated from the single V. shuttleworthii library. Comparative genomics analysis between V. shuttleworthii and V. vinifera grapes was conducted. When V. shuttleworthii EST’s were compared to the V. vinifera EST Unigene set, 2,140 unigenes from V. shuttleworthii did not find homologies (E-value '1E-20). Functional annotation indicated that a high percentage of these unmatched V. shuttleworthii ESTs are associated with disease resistance /stress tolerance. These genetic markers are being used to screen each years crosses of grape seedlings with increased disease, and insect resistance. Selected grape seedlings are now in the field undergoing fruit quality, yield, and disease resistance evaluations.