Skip to main content
ARS Home » Research » Publications at this Location » Publication #192980
Title: BETA VULGARIS ROOT ESTS MODULATED BY SUGAR BEET ROOT MAGGOT FEEDING: THEIR REGULATION BY WOUNDING AND DEFENSE SIGNALS AND POTENTIAL ROLE FOR INSECT CONTROL

Author
item Puthoff, David
item IVIC-HAYMES, SNEZANA - TOWSON UNIV TOWSON MD
item ZUZGA, SABINA - WARSAW POLAND
item Smigocki, Anna

Submitted to: National Academy of Sciences Sackler Symposium, From Functional Genomics of Model Organisms to Crop Plants for Global Health
Publication Type: Abstract Only
Publication Acceptance Date: 1/20/2006
Publication Date: 1/20/2006
Citation: Puthoff, D.P., Ivic-Haymes, S., Zuzga, S., Smigocki, A.C. 2006. Beta vulgaris root ests modulated by sugar beet root maggot feeding: their regulation by wounding and defense signals and potential role for insect control. National Academy of Sciences Sackler Symposium, From Functional Genomics of Model Organisms to Crop Plants for Global Health. p. 36.

Interpretive Summary:

Technical Abstract: The most devastating insect pest in U.S. sugar beet (Beta vulgaris L.) production is the sugar beet root maggot (SBRM, Tetanops myopaeformis Röder). SBRM-infested root and hypocotyl tissues from a moderately resistant (F1016) and susceptible ( F1010) cultivar were collected at 24 and 48 h to identify genes that are regulated by SBRM feeding using the Suppressive Subtractive Hybridization (SSH) enrichment method. Over 1000 cDNA clones were isolated and approximately half of them were confirmed to be differentially expressed in response to SBRM infestation using differential hybridization. Based on sequence analysis, more than 160 unique genes were identified, the majority of which belong to the defense and stress related functional classes. A set of the unique clones, most of which were from F1016, was used to construct macroarrays in order to examine gene expression profiles induced by wounding and treatment with the signaling molecules methyl jasmonate, (MJ), salicylic acid (SA) and ethylene. MJ treatment regulated the expression of 39% of the clones while SA and ethylene regulated only 19 and 11%, respectively, suggesting MJ signal transduction pathways may control sugar beet responses to SBRM feeding. Fusion of potential resistance genes with root-specific promoters will provide for targeted high levels of gene expression to the site of insect attack. Knowledge of plant-insect interactions and root responses to insect infestations will provide tools needed for designing novel methods of insect control thus decreasing our reliance on chemical pesticides.