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Title: Isolation of highly pathogenic avian influenza (HPAI) H5N1 strains from wild birds in the epizootic outbreak on the Ubsu-Nur Lake and their incorporation to the Russian Federation State collection of viruses

Author
item LVOV, D - MOSCOW, RUSSIA
item PRILIPOV, A - MOSCOW, RUSSIA
item SHCHELKANOV, M - MOSCOW, RUSSIA
item DERYABIN, P - MOSCOW, RUSSIA
item GREBENNIKOVA, T - MOSCOW, RUSSIA
item FEDYAKINA, I - MOSCOW, RUSSIA
item GALEGOV, G - MOSCOW, RUSSIA
item SHILOV, A - MOSCOW, RUSSIA
item SADYKOVA, G - MOSCOW, RUSSIA
item LYAPINA, O - MOSCOW, RUSSIA
item PEIRIS, M - HONG KONG SAR, CHINA
item Suarez, David

Submitted to: Voprosy Virusologii
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/30/2006
Publication Date: 12/1/2006
Citation: Lvov, D.K., Prilipov, A.G., Shchelkanov, M.Y., Deryabin, P.G., Grebennikova, T.V., Fedyakina, I.T., Galegov, G.A., Shilov, A.A., Sadykova, G.K., Lyapina, O.V., Peiris, M., Suarez, D.L. 2006. Isolation of highly pathogenic avian influenza (HPAI) H5N1 strains from wild birds in the epizootic outbreak on the Ubsu-Nur Lake and their incorporation to the Russian Federation State collection of viruses. Voprosy Virusologii. 51:14-18.

Interpretive Summary: Highly pathogenic avian influenza, or “Bird Flu”, has become a problem in many countries in the last few years. The virus causes a severe disease in chickens and other poultry . In recent months wild birds have also been infected with highly pathogenic avian influenza, which is extremely unusual. This paper describes the isolation and characterization of an H5N1 virus from wild birds and a domestic duck from Russia. The virus had all the indications that it was a highly pathogenic virus and the sequence showed it to be similar to other recent wild bird isolates from China. This paper provides evidence of the importance of wild birds in maintaining or spreading this outbreak of H5N1 avian influenza.

Technical Abstract: Complete genomes of two highly pathogenic avian influenza (HPAI) A (H5N1) strains isolated from wild bird Grebe (Podiceps cristatus) and domestic duck during an epizootic in summer 2005 in the same habitat of Western Siberia were analyzed. These strains had amino acid motif in the hemagglutinin cleavage site characteristic of HPAI. They were closely related in all genes to the H5N1 viruses isolated from wild birds in Qinghai Lake, China, in May 2005. Both Siberian viruses had Lys-627 in PB2, Glu-92 in NS1, Ser-31 in M2 and a 20-mer deletion in the NA gene (so belong to genotype Z). Both isolates were sensitive to remantadine, amantadine, ribavirin, arbidol in porcine embryo kidney cell line (PS) and replicated without trypsin upto 4.0-7.0 TCID50/ml in other cell line of mammalian origin: green monkey kidney (Vero-E6), human embryo lung (LEH), hamster kidney (BHK-21), canine kidney (MDCK).