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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #193328
Title: MOLECULAR MAPPING OF MALE-STERILITY LOCI MS2, AND MS9 IN SOYBEAN

Author
item CERVANTES-MARTINEZ, M - ISU
item XU, M - ISU
item ZHANG, L - ANHUI ACAD AG SCI
item HUANG, Z - ANHUI ACAD AG SCI
item KATO, K - OBIHIRO UNIVERSITY
item HORNER, H - ISU
item PALMER, REID

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/9/2006
Publication Date: 2/6/2007
Citation: Cervantes-Martinez, M., Xu, M., Zhang, L., Huang, Z., Kato, K.K., Horner, H.T., Palmer, R.G. 2007. Molecular mapping of male-sterility loci ms2, and ms9 in soybean. Crop Science. 47:374-379.

Interpretive Summary: Most plants have different traits or characteristics that can be passed on from parents to offspring. Some of these traits can be used by plant breeders to improve the plant, either directly, e.g. disease resistance or indirectly, e.g. by use of an improved breeding method. Male-sterile, female-fertile plants are useful in plant breeding and these fertility/sterility traits are inherited. These male-sterile, female-fertile plants, with pollinator insects, can be used to produce large quantities of hybrid seed for agronomic evaluations. Our objective was to determine the location of three male-sterile, female-fertile traits in soybean using molecular genetic methods. We would produce a 'roadmap', a genetic map showing the locations of the fertility/sterility traits. Our genetic studies indicated that a previously uncharacterized male-sterile, female-fertile trait was the same as male-sterile 2 (ms2). These two identical male-sterile, female-fertile traits were located on the soybean map in Group O. The third male-sterile, female-fertile mutant was located on the soybean map in Group N. The location of these two different traits can be used to place other traits in close physical proximity, so that they tend to be associated, i.e. physically (genetically) linked. These two traits can be treated as one trait in most situations and 'moved' by breeders more efficiently in soybean cultivar improvement programs. Improved cultivars benefit the seed producer (company), the farmer, and ultimately the consumer, through lower costs because of greater efficiency by the plant breeder.

Technical Abstract: Identifying molecular and/or phenotypic markers linked to male-sterile, female-fertile genes on the soybean [Glycine max (L.) Merr.] molecular map would facilitate early identification of male-sterile plants in breeding. The objective was to verify the chromosome location of the ms2 (Beeson) mutation, to determine the allelism and the chromosome location of the suspected ms2 (A00-39) mutation, and to determine the chromosome location of the ms9 (T359) mutation. Segregating F2 populations were identified from crosses of ms2ms2 (Beeson) x Minsoy (PI 27890), ms?ms?(A00-39) x Minsoy, and Minsoy x Ms9ms9 (T359H). All F2 plants were screened using single sequence repeat markers. Because the ms2 gene previously was localized on Classical Linkage Group 15, [Molecular Linkage Group (MLG) O], only 20 markers were selected. A total of 229 markers were selected for ms9, using as criteria for selection an approximate distance of less than 25 cM between two adjacent markers. The ms2 (Beeson) locus was located at 9.86 cM distance from the marker Sat_190 at the end of MLG O. The ms? (A00-39) locus was located on MLG O between markers Sat_190 and Scaa001, with a distance of 6.87 and 8.99 cM, respectively. The ms9 locus was located on MLG N between markers Satt521 and Satt237, with a distance of 8.5 and 16.2 cM, respectively. Classical allelism tests confirmed that mutant A00-39 was allelic to ms2 (Beeson). The A00-39 mutant line was assigned Genetic Type Collection number T375H and gene symbol Ms2ms2 (Ames 2). Thus Genetic Type T360H, previously identified at Ames, Iowa, becomes Ms2ms2 (Ames 1).