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Title: BIVALENT VACCINE CANDIDATE EXPRESSING ANTIGENS OF AVIAN INFLUENZA A (H5N1) AND NEWCASTLE DISEASE VIRUSES

Author
item STEEL, JOHN - MT. SINAI-NEW YORK, NY
item SOLORZANO, ALICIA - MT. SINAI-NEW YORK, NY
item GARCIA-SASTRE, ADOLFO - MT. SINAI-NEW YORK, NY
item Swayne, David
item PALESE, PETER - MT. SINAI-NEW YORK, NY

Submitted to: International Conference on Negative Strand Viruses
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2006
Publication Date: 6/17/2006
Citation: Steel, J., Solorzano, A., Garcia-Sastre, A., Swayne, D.E., Palese, P. 2006. Bivalent vaccine candidate expressing antigens of avian influenza A (H5N1) and Newcastle disease viruses [abstract]. Abstracts of the 13th International Conference on Negative Strand Viruses. p. 21.

Interpretive Summary:

Technical Abstract: The emergence and subsequent pan-continental spread of highly pathogenic avian influenza A (HPAI) (H5N1) viruses, with associated infection of poultry constitutes an unparalleled burden on the global poultry industry. Newcastle disease virus (NDV), a non-segmented negative strand RNA virus, is an avian pathogen which causes major economic losses. The cost of vaccination, recognised as the prime strategy for the protection of poultry from disease associated with these viruses, may be lowered through the use of a single multivalent vaccine. By reverse genetics techniques, we have generated a recombinant influenza virus expressing: (i) the hemagglutinin protein of influenza A/Vietnam/1203/04 (H5N1) modified by the removal of the polybasic cleavage peptide; and (ii) the ectodomain of the NDV hemagglutinin – neuraminidase (HN) protein in the place of the ectodomain of the influenza neuraminidase. The virus was efficiently propagated in 10 day old embryonated chicken eggs, and gel electrophoresis of viral RNA established the presence of all eight viral segments. The identities of the HN and HA encoding segments were confirmed by sequencing of RT-PCR products, and protein expression from these RNAs was demonstrated through gel electrophoresis of purified virion protein and immunofluorescence of infected cells. We propose that this bivalent virus may have potential as a live bivalent avian influenza and Newcastle disease virus vaccine.