Author
Kraus, Jennifer | |
PUTNAM, M - OREGON STATE UNIVERSITY | |
Martin, Robert |
Submitted to: Phytopathology
Publication Type: Abstract Only Publication Acceptance Date: 4/4/2006 Publication Date: 5/1/2006 Citation: Kraus, J.E., Putnam, M., Martin, R.R. 2006. Characterization of viruses in verbena x hybrida. Phytopathology. 96:S36. Interpretive Summary: Technical Abstract: Viruses in Verbena x hybrida cultivars have been a sporadic but chronic problem in the floriculture industry. Affected plants can develop symptoms (mosaic, necrosis, chlorosis, reduced vigor) and are not saleable. We solicited symptomatic plants and new cultivars from growers then surveyed by group-specific RT/PCR (AGDIA, Elkhart IN) and double-stranded RNA analysis. Four symptomatic cultivars were found to contain viuses. ‘Lavender Shades’ was found to contain Bean Yellow Mosaic Potyvirus (reported previously) and a '10kb dsRNA. ‘Silver Ann’ tested positive for both Potexvirus and Potyvirus and had a complex dsRNA profile. ‘Taylor Town Red’ tested positive for Potyvirus and contained a '10kb dsRNA. ‘ES Burg 1136-3' had reduced vigor, tested positive for Trichovirus , and contained '7.5 and '6.7 kb dsRNAs. ‘Lanai Scarlet 921-1' and several other cultivars were symptomless but tested positive for Carmovirus and had a dsRNA profile consistent with that of Carmoviruses. The BYMV in ‘Lavender Shades’ was most readily detected in terminal leaf tips. An RT/PCR assay was developed and used to show that BYMV is more readily detected in young plants grown in soil than in tissue-cultured plants or older plants. Characterization of the viruses is ongoing. |