Author
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ROBERTSON, NANCY |
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Submitted to: Phytopathology
Publication Type: Abstract Only Publication Acceptance Date: 5/16/2006 Publication Date: 6/20/2006 Citation: Robertson, N.L. 2006. A novel virus in Angelica lucida (wild celery) in Alaska. Phytopathology. 96(6):31. Interpretive Summary: Technical Abstract: Documentation of viruses in native plants is scarce despite the fact that affected plants may act as hosts for agronomical important viruses and impact natural ecosystems. In south central Alaska, wild celery (Angelica lucida, family Apiaceae) plants growing along roadsides near crops and in forests contained severe leaf mottling and vein-clearing. Leaves were collected and processed immediately or stored at -80º C. Virions were extracted, partially purified, and serologically assayed using universal potyvirus antiserum (Agdia, Inc.). Confirmation of a plant virus belonging to the family Potyviridae was by ELISA and detection of a coat protein ~35 kDa by Western analysis. PCR assays using a universal potyvirus primer set that targeted the coat protein and polymerase genes, generated the predicted ~ 2K nt fragment. A Blast search of nucleotide sequences obtained from the fragments determined that although the sequence was most similar to several potyvirus that may also may affect plant species in the family Apiaceae (Carrot virus Y, Apium virus Y, and Turnip mosaic virus), it was unique enough to be placed as a novel virus in the family Potyviridae, and tentatively named Angelica virus Y (AnVY). Although A. lucida is the only known natural host to AnVY, it is important to determine if this new virus is a potential threat to Alaska vegetable crops. |
