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ARS Home » Research » Publications at this Location » Publication #195048
Title: DIFFERENTIAL EXPRESSION OF PAPAYA PROTEIN PROFILE USING PROTEOMIC TOOLS

Author
item PAIDI, MAYA - UNIV HAWAII
item LEE, S - UNIV HAWAII
item LI, Q - UNIV HAWAII
item Moore, Paul
item ZHU, Y - HARC

Submitted to: CTAHR Student Research Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 3/3/2006
Publication Date: 4/7/2006
Citation: Paidi, M., Lee, S.E., Li, Q., Moore, P.H., Zhu, Y.J. 2006. Differential Expression of Papaya Protein Profile Using Proteomic Tools. 18th Annual CTAHR Student Research Symposium. (Abstracts)#21, Pg. 21.

Interpretive Summary:

Technical Abstract: The objective of our project is to evaluate the potential for using proteomic approaches to discover the basis for differential susceptibility of Hawaii papaya cultivars to the root rot pathogen Phytophthora palmivora. Based on previous studies showing that Kapoho was the most resistance cultivar and Sunup was a susceptible one, we selected these two lines for a comparative root protein analysis. Soluble proteins were extracted from 3-month old plants using phenol-SDS extraction buffer. Approximate 200 micrograms of protein was resolved by two-dimensional electrophoresis (2-DE). Because plant tissues contain many compounds that interfere with the sensitive 2D gels, extraction procedures were validated before attempting protein separations. We ran our preliminary studies in replicates of three 2D gels. The gels were stained in Coomassie Blue and spots were analyzed using Biorad PDQuest 8.0 software. More reliable 2-DE gels were achieved by using an improved phenol-SDS extraction method. Over 300 protein spots were compared for differences in abundance between Sunup and Kamiya. Significantly over-expressed protein spots were identified from each cultivar. Further studies will characterize and identify these proteins and determine their possible role in disease resistance. This project will contribute toward the identification and characterization of proteins that are differentially expressed between the resistant cultivar and susceptible cultivars to provide a systematic approach to elucidate the mechanisms of phytophthora resistance.