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Title: VARIATION OF MICROSATELLITE MARKERS AMONG SELECTED PAPAYA VARIETIES

Author
item EUSTICE, M - UNIV HAWAII
item LAI, C - UNIV HAWAII
item YU, Q - HARC
item PRESTING, G - UNIV HAWAII
item Moore, Paul
item MING, R - UNIV ILLINOIS

Submitted to: CTAHR Student Research Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 3/3/2006
Publication Date: 4/7/2006
Citation: Eustice, M., Lai, C.W., Yu, Q., Presting, G., Moore, P.H., Ming, R. 2006. Variation of microsatellite markers among selected papaya varieties. 18th Annual CTAHR Student Research Symposium. (Abstracts)#77, pg. 49.

Interpretive Summary:

Technical Abstract: Microsatellites, or simple sequence repeats (SSRs), are simple, tandemly repeated mono- to hexa-nucleotide sequence motifs. Due to their co-dominant nature, ability to detect high levels of allelic diversity, and ubiquitous distribution, they have been widely used as genetic markers. Genomic research on papaya (Carica papaya L.) is rapidly advancing and has recently yielded significant quantities of sequence data. We used 1.3 mega base (Mb) random genomic sequences, 17.1 Mb bacterial artificial chromosome end sequences (BES), and 1.6 Mb of cDNA sequences for mining microsatellites. A total of 919 SSR markers has been identified with sufficient flanking sequences to permit amplification. The SSRs were screened with parents of five mapping populations to test for polymorphism. The highest level of polymorphism, with 301 polymorphic markers, was between the Hawaii cultivar, SunUp, and an unimproved Israeli variety, AU9. UH928 and UH918, two unimproved varieties, crossed with SunUp followed close behind with 248 and 239 polymorphic markers, respectively. Next lower in polymorphism, with 175 polymorphic markers, was 2H94, a small fruit mutant, crossed with Khak dam, a Thai variety. The lowest level of polymorphism, with 165 polymorphic markers, was between SunUp and Kapoho, two closely related varieties. The level of polymorphism among the five parental varieties was highest between the unimproved germplasm and the cultivated varieties, reflecting the relatedness of the varieties tested. The availability of these SSRs will greatly assist in the genetic and physical mapping of the papaya genome as well as enhance the capacity for papaya improvement.