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Title: MAPPING PHYSIOLOGICAL TRAITS IN CARICA PAPAYA USING MICROSATELLITE MARKERS

Author
item VEATCH, O - UNIV HAWAII
item YU, Q - HARC
item EUSTICE, M - UNIV HAWAII
item Moore, Paul
item PAULL, R - UNIV HAWAII
item MING, R - UNIV ILLINOIS

Submitted to: CTAHR Student Research Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 3/3/2006
Publication Date: 4/7/2006
Citation: Veatch, O.J., Yu, Q., Eustice, M., Moore, P.H., Paull, R., Ming, R. 2006. Mapping physiological traits in Carica papaya using microsatellite markers. 18th Annual CTAHR Student Research Symposium [abstract]. Paper No. 30-25.

Interpretive Summary:

Technical Abstract: Different varieties of papaya (Carica papaya L.) vary in the phenotypic expression of agronomically important traits. Genetic loci responsible for these differences can be mapped using DNA markers to genotype a segregating progeny population derived from a controlled cross between parents having different phenotypes. Two unique varieties of papaya, Khak Dam and 2H94, were chosen as parental materials based on their widely varied phenotypic expression of fruit size, shape, and flesh color. These varieties were used to produce an F2 population that was surveyed for DNA polymorphisms using microsatellite markers. The 213 F2 individuals were scored for flesh color and fruit weight, length, and width. Fruit phenotype data was analyzed by constructing histograms to assess the distribution of these traits. Flesh color segregated in a 3:1 ratio with yellow flesh color being dominant suggesting that color is a single gene controlled trait. Normal distribution was detected for length and width suggesting quantitative inheritance for these traits. Fruit weight segregated as a mix of normal and bimodal distribution suggesting involvement of a major gene modified by quantitative trait loci (QTL). A total of 919 microsatellite markers screened for polymorphisms between parents showed 175 of them to be polymorphic. These markers will be used to construct a genetic linkage map to identify major gene loci and QTLs controlling these traits. Sequence specific DNA markers linked to these target traits will be developed for marker- assisted selection.