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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #197100
Title: DETECTION OF CANDIDATUS LIBERIBACTER ASIATICUS FROM CITRUS HUANGLONGBING SAMPLES IN CHINA BY NESTED CONVENTIONAL AND REAL-TIME PCR

Author
item Chen, Jianchi
item DENG, X - S CHINA AGR UNV-GUANGZHOU
item ZHOU, G - S CHINA AGR UNV-GUANGZHOU
item FENG, Z - S CHINA AGR UNV-GUANGZHOU
item XU, J - S CHINA AGR UNV-GUANGZHOU
item LIU, Q - S CHINA AGR UNV-GUANGZHOU
item Civerolo, Edwin

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/15/2006
Publication Date: 7/29/2006
Citation: Chen, J., Deng, X.L., Zhou, G., Feng, Z., Xu, J.H., Liu, Q.G., Civerolo, E.L. 2006. Detection of candidatus liberibacter asiaticus from citrus huanglongbing samples in china by nested conventional and real-time pcr. [abstract]. Phytopathology. 96:S23

Interpretive Summary:

Technical Abstract: Candidatus Liberibacter asiaticus is one of the three known bacterial Candidatus species causing citrus Huanglongbing (HLB) or yellow shoot disease. HLB is highly destructive in citrus cultivation and is currently known to presence in Asia, Africa, South and North America. Pathogen detection plays a key role in HLB control. Because of the uncultureable status, C. L. species is currently defined by the characteristic of 16S rDNA sequence. In this study, HLB was first recognized by symptomatology. C. L. asiaticus was detected by PCR from DNA extracted from diseased citrus tissue. We experienced that low bacterial titer and possible inhibitors from plant tissue were the challenge for quick and accurate PCR detection. We overcome this problem by a PCR using the specific primer set OI1 and OI2c nested with a primer set general to bacterial 16S rDNA. This nested PCR format was also performed on a SYBR green based real-time PCR with comparable detection sensitivity and accuracy, but eliminated the post-PCR agarose gel electrophoresis process.