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Title: GENETIC DIVERSITY OF SORGHUM ACCESSIONS RESISTANT TO GREENBUGS AS ASSESSED WITH AFLP MARKERS

Author
item WU, Y - DEPT OF FORESTRY, OSU
item Huang, Yinghua
item TAUER, C - DEPT OF FORESTRY, OSU
item Porter, David

Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/2/2005
Publication Date: 1/15/2005
Citation: Wu, Y.Q., Huang, Y., Tauer, C., Porter, D.R. 2005. Genetic diversity of sorghum accessions resistant to greenbugs as assessed with AFLP markers [abstract]. In: Proceedings of the XIII Annual International Plant & Animal Genome Conference, January 15-19, 2005, San Diego, California. p. 119.

Interpretive Summary:

Technical Abstract: Sorghum, Sorghum bicolor (L.) Moench, is the fifth important cereal crop grown worldwide and the fourth in the USA. Greenbug, Schizaphids graminum Rondani, has been a major insect pest of sorghum with several biotypes reported. Greenbug biotype I is prevalent attackers and most virulent on sorghum plants. Breeding for resistance is an effective way in control of greenbug damages. A successful breeding program relies upon a better understanding of the breeding materials originated from diverse sources. However, the genetic diversity and relatedness among the greenbug biotype I resistant accessions collected from different geographic origins have not been well characterized, although a rich germplasm collection is available. In our current study, twenty-six sorghum accessions from twelve countries were evaluated for both resistance to greenbug biotype I and genetic diversity using florescence labeled amplified fragment length polymorphism (AFLP). Twenty-six AFLP primer combinations produced 819 polymorphic fragments indicating a relative high level of polymorphism among accessions. Genetic similarity coefficients among the sorghum accessions ranged from 0.69 to 0.90. Cluster and principal component analyses indicated that there were several distinct groups based on polymorphic bands. This study has allowed the identification of new genetic sources with substantial genetic variation and distinct groupings of resistant accessions that have the potential for the development of durable greenbug resistant sorghum.