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Title: INTEGRATION OF MAREK'S DISEASE VIRUS INTO THE CHICKEN GENOME

Author
item REDDY, S - TEXAS A & M
item DRECHSLER, Y - TEXAS A & M
item SUCHODOLSKI, P - TEXAS A & M
item RAUDSEPP, T - TEXAS A & M
item LUPIANI, B - TEXAS A & M
item CHOWDHARY, B - TEXAS A & M
item Lee, Lucy

Submitted to: Workshop on Molecular Pathogenesis of Marek's Disease and Avian Immunology
Publication Type: Abstract Only
Publication Acceptance Date: 8/5/2006
Publication Date: 8/5/2006
Citation: Reddy, S., Drechsler, Y., Suchodolski, P., Raudsepp, T.B., Lupiani, B., Chowdhary, B.P., Lee, L.F. 2006. Integration of Marek's disease virus into the chicken genome [abstract]. 4th International Workshop on Molecular Pathogenesis of Marek's Disease Virus. p. 52.

Interpretive Summary:

Technical Abstract: Marek's disease virus (MDV) is associated with rapid onset of transformation of T-lymphocytes in infected chickens. Delecluse and Hammerschmidt (1993) have shown that MDV genome is integrated in MDV induced lymphoma cell lines. They have also shown that latent MDV could be reactivated from chromosomally integrated state. We do not completely understand the role of MDV integration in latency and transformation. We have investigated MDV integration in several transformed cell lines derived from chicken using fluorescence in situ hybridization (FISH). Integration patterns in different cell lines varied suggesting that it may be a random insertion, but most of the integrations were confined to the telomer regions of the chromosomes. We are currently using inverse PCR to identify the location of these integration sites.