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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Crop and Commodity Protection Research » Research » Publications at this Location » Publication #197941
Title: SPECTROFLUOROMETRIC AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF a-TOCOPHEROL ACETATE IN OLIVE OIL

Author
item CHO, IL KYU - KOSEF
item RIMA, JAMIL - KOSEF
item Chang, Chiou

Submitted to: Journal of Food Composition and Analysis
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/20/2006
Publication Date: 2/1/2007
Citation: Cho, I., Rima, J., Chang, C.L. 2007. Spectrofluorometric and high-performance liquid chromatographic determination of a-tocopherol acetate in olive oil. Journal of Food Composition and Analysis. 20:57-62

Interpretive Summary: HPLC withUV, fluorimetric and Spectorfluorimetic and internal addition standard methods used to quantify the '-tocopherol acetate level in olive oil.

Technical Abstract: A high performance liquid chromatographic (HPLC) method was developed for the quantitative determination of '-acetate tocopherol in olive oil. After extracts in n-hexane, acetate '- tocopherol were quantitatively analyzed by HPLC with fluorimetric detector. The presence of acetate '- tocopherol in olive oil is further confirmed with HPLC-UV and fluorescence detection. A spectrofluorimetric and internal addition standard methods are also used to quantify acetate '- tocopherol in olive oil. An internal standard method is based on a known concentration of acetate '- tocopherol that is added in every sample that is analyzed. Acetate ' - tocopherol levels as determined in samples by HPLC with UV and fluorescence detection is 30 ± 0.5 mg/kg. It did not differ significantly from the levels determined by spectrofluromiter (30 ± 0.5 mg/kg). Standards were checked for linearity giving correlation coefficients that were higher than 0.99 in the concentration range of 1 and 6 'molL-1