CPG OLIGODEOXYNUCLEOTIDES-MEDIATED IMMUNOMODULATION OF MIC2 RECOMBINANT VACCINE-INDUCED PROTECTION AGAINST COCCIDIOSIS

Authors: DALLOULA, RAMI - VISITING SY; Lillehoj, Hyun; KLINMAN, DENNIS - USFDA

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/30/2006
Publication Date: 10/21/2006
Citation: Dalloula, R.A., Lillehoj, H.S., Klinman, D.M. 2006.Cpg oligodeoxynucleotides-mediated immunomodulation of mic2 recombinant vaccine-induced protection against coccidiosis. Proceedings of International Avian Immunology Research Group Meeting. Oct. 21-24. Paris, France. P 99.

Interpretive Summary:

Technical Abstract: Recent studies form our laboratory demonstrated that short oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs) exert a positive effect on weight loss and oocyst shedding associated with Eimeria infection when injected in vivo. The present work investigated the effects of CpG ODNs on embryo vaccination with recombinant microneme protein (MIC2) and on live challenge infecton with Eimeria. In ovo injection of CpG ODNs alone enhanced resistance to E. acervulina infection as best exemplified by reduced oocyst shedding. Two new CpG ODNs reduced the oocyst load, but did not affect weight gain. When co-administered with the recombinant microneme protein, both ODNs reduced oocyst shedding; however, only ODN D19 plus MIC2 consistently improved weight gain. Vaccinating with ODN 2006 or MIC2 protein curtailed oocyst shedding but did not enhance weight gain in Eimeria tenella-infected birds. Co-administration of CpG ODN and MIC2 did not have an additive effect in reducing the oocyst output; however, it resulted in the highest and lowest Ab response before and after Eimeria tenella infection, respectively. Collectively, CpG ODNs administered in ovo demonstrated immunoenhancing and adjuvant effects following live challenge infection with E. acervulina infection.