Author
 |
Yeh, Hung-Yueh |
|
Klesius, Phillip |
|
Submitted to: Genbank
Publication Type: Other Publication Acceptance Date: 8/10/2006 Publication Date: 8/22/2006 Citation: Yeh, H., Klesius, P.H. 2006. Molecular cloning and expression of channel catfish, Ictalurus punctatus, complement membrane attack complex inhibitor CD59. Genbank. Interpretive Summary: Technical Abstract: The channel catfish, Ictalurus punctatus, complement membrane attack complex inhibitor CD59 gene was cloned and analyzed. Total RNA from tissues was isolated and cDNA libraries were constructed by the rapid amplification cDNA end (RACE) method. The gene-specific primers in conjunction with the RACE primers were used to PCR amplify 5’- and 3’-ends of the CD59 transcript. The complete channel catfish CD59 cDNA comprised 1109 bp including a 132-bp 5’-untranslated, a 360-bp open reading frame and a 617-bp 3’-untranslated regions. The open reading frame encodes a putative protein of 119 amino acid residues with calculated molecular mass (without potential glycosylation) of 13.2 kDa. |
