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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #198845

Title: The Nucleotide Sequence Of Strawberry Chlorotic Fleck Associated Virus, A Novel Closterovirus

Author
item TZANETAKIS, I - OREGON STATE UNIVERSITY
item Martin, Robert

Submitted to: Panhellenic Phytopathogical Conference
Publication Type: Abstract Only
Publication Acceptance Date: 10/10/2006
Publication Date: 10/16/2006
Citation: Tzanetakis, I.E., Martin, R.R. 2006. The nucleotide sequence of Strawberry chlorotic fleck associated virus, a novel closterovirus. Panhellenic Phytopathogical Conference.

Interpretive Summary:

Technical Abstract: Chlorotic fleck, a strawberry disease caused by a graft and aphid transmissible agent(s), was identified more than 45 years ago. The mode of transmission indicated that a virus may be associated with symptoms. A novel closterovirus, in addition to other known strawberry viruses, was identified in the plant when double-stranded RNA was extracted from a chlorotic fleck disease positive clone. Sequence analysis of the virus revealed the close relationship of the novel virus with members of the Closterovirus genus, the aphid-transmitted closteroviruses, in accordance with the aphid transmissibility of the chlorotic fleck agent. The complete nucleotide sequence of the virus has been determined. The genome organization is similar to Beet yellows virus but its sequence is most closely related to Citrus tristeza virus. Several hundred plants have been tested for the presence of the virus in areas of high aphid incidence with only one identified infected with it. We are currently testing several aphid species for their ability to transmit the virus.