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Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 8/11/2006 Publication Date: 8/13/2006 Citation: Liu, Y. 2006. Diagnostic Methods for Food-Borne Pathogen Detection and Antimicrobial Resistance. Meeting Abstract. S02. pg. 62. Interpretive Summary: Technical Abstract: Accurate and rapid detection of pathogens and their antimicrobial resistance is an important tool for ensuring public health. Antimicrobial resistance detection can be classified into phenotypic methods and genotypic methods. In contrast to the phenotypic methods that are laborious and time-consuming, genotypic methods provide rapid analysis time and better sensitivity. DNA microarray assays were developed for rapid identification of different serogroups of E. coli (O7, O111, O104 and O157). Both oligonucleotides (35-mers) and PCR fragments from the O-antigen gene clusters of different serogroups were selected as targets. The long PCR products obtained from the regions flanking the O-antigen gene clusters were labeled with fluorescence dye and were hybridized to the targets. Our results demonstrated that both oligonucleotides (35-mers) and PCR fragments generated specific signals for each serogroup. This report not only represented the first report for E. coli serotyping using microarray technology, but also demonstrated the potential application of DNA microarrays for the identification of bacterial pathogens and for assessing their antimicrobial resistance potential. |
