Author
WANG, QIQUAN - UNIVERSITY OF CALIFORNIA | |
Yates, Scott |
Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only Publication Acceptance Date: 9/10/2006 Publication Date: 9/14/2006 Citation: Wang, Q., Yates, S.R. 2006. Analysis of manure-derived oxytetracycline in amended soil. American Chemical Society National Meeting. pp# AGRO-5 Interpretive Summary: Technical Abstract: As a member of tetracycline antibiotic family, oxytetracycline is widely administered to animals. With the application of manure from medicated animals as fertilizer into agricultural land, oxytetracycline may enter the environment. For studying oxytetracycline contamination and its fate in agricultural soil, an analytical method was developed to analyze oxytetracycline in manure amended soils. Sixteen grams of manure-amended soil was extracted with 2.5 g citric acid, 1.5 oxalic acid, and 15 mL methanol:water = 90:10 solution and extraction was repeated for a total of 3 times. Extract was directly analyzed with high performance liquid chromatography. The extraction recovery of the developed method showed to be much higher than those with 2% HCl methanol or pH = 4 EDTA-McIlvaine buffer. Effect of manure amended ratio, manure type, and soil type on extraction recovery was investigated. Detection limit, linearity, and specificity of the developed method were also studied |