Egg embryo development detection with hyperspectral imaging

Authors: Lawrence, Kurt; Smith, Douglas; Windham, William; Heitschmidt, Gerald - Jerry; Park, Bosoon

Submitted to: Proceedings of SPIE
Publication Type: Proceedings
Publication Acceptance Date: 9/11/2006
Publication Date: 10/25/2006
Citation: Lawrence, K.C., Smith, D.P., Windham, W.R., Heitschmidt, G.W., Park, B. 2006. Egg embryo development detection with hyperspectral imaging. Proceedings of SPIE. 6381.

Interpretive Summary: The U.S. egg industry incubates anywhere from 130 million to over one billion infertile eggs each year. Some of these infertile eggs explode in the hatching cabinet and can potentially spread molds or bacteria to all the eggs in the cabinet. Hatching cabinet energy and space are also wasted on these non-developing eggs. A method to detect the embryo development of incubated eggs was developed. The method used a technology called hyperspectral imaging to image the light transmitting through an egg. This light, from the visible and near infrared region of the spectrum, is then averaged over the egg, and a model was created to predict embryo development from day 0 to day 3 of incubation. The method was able to predict embryo development after only one day of incubation with 100% accuracy on a limited number of eggs. Further experiments with large egg populations from different flocks are planned to validate and verify these results.

Technical Abstract: In the U. S. egg industry, anywhere from 130 million to over one billion infertile eggs are incubated each year. Some of these infertile eggs explode in the hatching cabinet and can potentially spread molds or bacteria to all the eggs in the cabinet. A method to detect the embryo development of incubated eggs was developed. Twelve brown-shell hatching eggs from two replicates (n=24) were incubated and imaged to identify embryo development. A hyperspectral imaging system was used to collect transmission images from 420 to 840 nm of brown-shell eggs positioned with the air cell vertical and normal to the camera lens. Raw transmission images from about 400 to 900 nm were collected for every egg on days 0, 1, 2, and 3 of incubation. A total of 96 images were collected and eggs were broken out on day 6 to determine fertility. After breakout, all eggs were found to be fertile. Therefore, this paper presents results for egg embryo development, not fertility. The original hyperspectral data and spectral means for each egg were both used to create embryo development models. With the hyperspectral data range reduced to about 500 to 700 nm, a minimum noise fraction transformation was used, along with a Mahalanobis Distance classification model, to predict development. Days 2 and 3 were all correctly classified (100%), while day 0 and day 1 were classified at 95.8% and 91.7%, respectively. Alternatively, the mean spectra from each egg were used to develop a partial least squares regression (PLSR) model. First, a PLSR model was developed with all eggs and all days. The data were multiplicative scatter corrected, spectrally smoothed, and the wavelength range was reduced to 539 - 770 nm. With a one-out cross validation, all eggs for all days were correctly classified (100%). Second, a PLSR model was developed with data from day 0 and day 3, and the model was validated with data from day 1 and 2. For day 1, 22 of 24 eggs were correctly classified (91.7%) and for day 2, all eggs were correctly classified (100%). Although the results are based on relatively small sample sizes, they are encouraging. However, larger sample sizes, from multiple flocks, will be needed to fully validate and verify these models. Additionally, future experiments must also include non-fertile eggs so the fertile / non-fertile effect can be determined.