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ARS Home » Pacific West Area » Riverside, California » National Clonal Germplasm Repository for Citrus » Research » Publications at this Location » Publication #201432
Title: Complete Nucleotide Sequence of a New Genotype of Citrus Tristeza Virus from an Isolate Having a Mixed Infection

Author
item Keremane, Manjunath
item RAMADUGU, C. - UNIV OF CA, RIVERSIDE
item WARREN, C. - UNIV OF FL, GAINESVILLE
item NIBLETT, C. - UNIV OF FL, GAINESVILLE
item Lee, Richard

Submitted to: International Organization of Citrus Virologists Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/1/2006
Publication Date: 1/10/2007
Citation: Keremane, M.L., Ramadugu, C., Warren, C., Niblett, C.L., Lee, R.F. 2007. Complete Nucleotide Sequence of a New Genotype of Citrus Tristeza Virus from an Isolate Having a Mixed Infection. International Organization of Citrus Virologists Proceedings, 16th Conference, Pg. 487.

Interpretive Summary: An isolate of Citrus tristeza virus (CTV) that causes severe stem pitting in grapefruits was used for sequencing. Analysis of the isolate revealed the presence of at least three different populations, one belonging to T30 genotype and the other two belonging to new genotypes, designated T2K and T38K. The complete sequence of the T2K genotype was determined. The genomes of T38K and T30 were partially sequenced. Analyses of these sequences reveal possible recombination events during the evolution of these genotypes. The possibilities of pseudo-recombinants resulting from “primer-independent reverse transcription” of CTV RNA are discussed.

Technical Abstract: An isolate of Citrus tristeza virus (CTV) that causes severe stem pitting in grapefruits (# 3800) was used for sequencing. Analysis of the isolate revealed the presence of at least three different populations, one belonging to T30 genotype and the other two belonging to new genotypes, designated T2K and T38K. The complete sequence of the T2K genotype was determined. The genomes of T38K and T30 were partially sequenced. Analyses of these sequences reveal possible recombination events during the evolution of these genotypes. The possibilities of pseudo-recombinants resulting from “primer-independent reverse transcription” of CTV RNA are discussed.