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ARS Home » Southeast Area » Fayetteville, Arkansas » Poultry Production and Product Safety Research » Research » Publications at this Location » Publication #203080
Title: Stimulation of Mucosal Immunity with Probiotics

Author
item HARGIS, BILLY - UNIVERSITY OF ARKANSAS
item FARNELL, MORGAN - TEXAS A&M
item Donoghue, Ann - Annie
item SOLIS DEL SANTOS, FASTO - UNIVERSITY OF ARKANSAS
item BLORE, PAM - UNIVERSITY OF ARKANSAS
item DONOGHUE, DAN - UNIVERSITY OF ARKANSAS
item TELLEZ, GUILLERMO - UNIVERSITY OF ARKANSAS

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/22/2006
Publication Date: 11/25/2006
Citation: Hargis, B.M., Farnell, M.B., Donoghue, A.M., Solis Del Santos, F., Blore, P.J., Donoghue, D.J., Tellez, G. 2006. Stimulation of Mucosal Immunity with Probiotics [abstract]. Arkansas Bioscience Institute Annual Meeting. p. 14.

Interpretive Summary:

Technical Abstract: The immune system of neonatal chicks is functionally immature during the first week of life. Researchers have previously demonstrated that the avian humoral response can be increased through the use of probiotics. Although the humoral response provides the chick with an effective mechanism to combat pathogens, sufficient antibody titers are not attained until 7 to 10 d post infection. However, the innate immune system (i.e. heterophils) can respond much more quickly to pathogens. The objective of this study was to determine whether probiotic bacteria can also up regulate heterophil function. Heterophils were isolated from the peripheral blood of neonatal chickens by using a discontinuous density gradient. Oxidative burst and degranulation are bactericidal mechanisms used by heterophils to kill pathogens and were used in this study as indicators of heterophil function. We found that each of the 10 “generally recognized as safe” (GRAS) probiotic isolates (designated G1-11) tested, in vitro, were capable of increasing heterophil oxidative burst and degranulation when compared to unstimulated controls. Bacillus subtilis (G3), Lactococcus lactis lactis (G6) and Lactobacillus acidophilus (G8) isolates were determined to elicit the greatest heterophil response in vitro and were subsequently fed to chicks. Phosphate buffered saline (PBS) or one of these three probiotic isolates (50 chicks/treatment) resuspended in PBS was administered by oral gavage on the day of hatch. Heterophils were isolated from chicks from each of these four treatment groups 24 h post treatment. Significant increases in heterophil degranulation and oxidative burst were observed with the G3, G6 and G8 treated chicks when compared to heterophils isolated from birds with no probiotic treatment. These data suggest that probiotic bacteria can significantly improve heterophil oxidative burst and degranulation in broilers. To our knowledge this is the first study demonstrating a relationship between probiotics and avian heterophil function.