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ARS Home » Midwest Area » Madison, Wisconsin » U.S. Dairy Forage Research Center » Research » Publications at this Location » Publication #203358
Title: DISSOLUTION OF THE ENTIRE PLANT CELL WALL FRACTION FOR SOLUTION-STATE NMR (AND CHEMOMETRICS)

Author
item Ralph, John
item LU, FACHUANG - UNIV. OF WISCONSIN-MADISO

Submitted to: Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/14/2006
Publication Date: 12/13/2006
Citation: Ralph, J., Lu, F. 2006. Dissolution of the entire plant cell wall fraction for solution-state NMR (and chemometrics). In: Proceedings of Fifth Umea-Imperial Minisymposium on Chemometrics and Metabonomics, December 13, 2006, Umea, Sweden, p. 5.

Interpretive Summary:

Technical Abstract: Although dissolution of intact cell walls is problematic, finely divided (ball-milled) walls are amenable to dissolution in two useful solvent systems, DMSO/N-methyl imidazole and DMSO/tetrabutyl ammonium fluoride. In the former system, acetylation is trivially accomplished, producing acetylated cell wall materials that are essentially fully soluble in CDCl3 for solution-state NMR. Exploiting the dispersion of 2D (and even 3D) NMR allows strikingly detailed structural analysis of the wall components without the need for isolation and fractionation. Furthermore, the structural “fingerprint” of the cell wall produced by 2D HSQC experiments is potentially unmatched by any other spectroscopy. Such dissolution/NMR methods therefore potentially lend themselves well to chemometrics methods, as are being explored here in Umeå.