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Title: Cross reaction of anti-human CD monoclonal antibodies on guinea pig cells: A summary of the guinea pig section of the HLDA8 Animal Homologues data

Author
item LASCO, TODD - CO STATE UNIV.
item GONZALEZ-JUARRERO, MERCEDES - CO STATE UNIV.
item SAALMUELLER, ARMIN - UNIV. VET MED AUSTRIA
item Lunney, Joan

Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/22/2006
Publication Date: 7/20/2007
Citation: Lasco, T., Gonzalez-Juarrero, M., Saalmueller, A., Lunney, J.K. 2007. Cross reaction of anti-human CD monoclonal antibodies on guinea pig cells: A summary of the guinea pig section of the HLDA8 Animal Homologues data. Veterinary Immunology and Immunopathology.119:131-6.

Interpretive Summary: There are numerous animal models of infectious diseases, yet for some animal species immune reagents are limited. The 8th International Workshop on Human Leukocyte Differentiation Antigens (HLDA8, Adelaide, Australia) sponsored Animal Homologues section. This section tested a panel of 377 commercially available monoclonal antibodies (mAbs), specific for a total of 144 human immune cell marker (or CD) antigens, for cross-reactivity studies in a range of 17 vertebrate species. This manuscript reports the testing of these mAbs for positive reactivity with guinea pig immune cells or splenocytes, as affirmed by immunostaining and flow cytometric analyses. In the first phase of this study only 36 of the total 367 mAbs (9.81%) were found to be cross-reactive with guinea pig immune cells surface molecules. The majority (26 of 36) of these cross-reactive mAbs were analyzed further to confirm appropriate cell subset expression by two-color immunofluorescence. The results indicated that 15 anti-human CD9, CD10, CD14, CD20 (2 clones), CD22, CD25, CD29 (2 clones), CD32, CD47 (2 clones), CD49d, CD49e and CD86 mAbs exhibit clear cross-reactivity with guinea pig splenocytes. These mAb can potentially be added to the limited repertoire of reagents available for studies in this model system, important particularly for respiratory infections such as tuberculosis. This data clearly indicates that cross-reactions have been defined for mouse anti-human CD mAb on guinea pig cells. Thus, one aim of this HLDA8 Animal Homologues section has been fulfilled, i.e., to identify mAbs which recognize conserved, species-independent CD epitopes. These results will contribute to the availability of mAbs and tools in veterinary medicine and immunology.

Technical Abstract: A panel of 377 commercially available monoclonal antibodies (mAbs) specific for a total of 144 CD antigens was submitted to the 8th International Workshop on Human Leukocyte Differentiation Antigens (HLDA8, Adelaide, Australia) for cross-reactivity studies in a range of vertebrate species. Each of the mAbs in this study was screened for positive reactivity with guinea pig splenocytes by flow cytometry. In the first phase of this study 36 of the total 367 mAbs (9.81%) cross-reacted with splenocyte surface molecules. The majority (26 of 36) of these cross-reactive mAbs were analyzed further to confirm appropriate cell subset expression by two-color immunofluorescence. Our results indicate that 15 anti-human CD9, CD10, CD14, CD20 (2 clones), CD22, CD25, CD29 (2 clones), CD32, CD47 (2 clones), CD49d, CD49e and CD86 mAbs exhibit clear cross-reactivity with guinea pig splenocytes. These mAb can potentially be added to the limited repertoire of reagents available for studies in this model system. This data clearly indicates that mouse anti-human CD mAb guinea pig cross-reactions have been defined and that an aim of this HLDA8 section has been fulfilled, i.e., to identify mAbs which recognize conserved, species-independent CD epitopes. These results will contribute to the availability of mAbs and tools in veterinary medicine and immunology.