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ARS Home » Midwest Area » West Lafayette, Indiana » Crop Production and Pest Control Research » Research » Publications at this Location » Publication #203656
Title: Development of a rapid detection method for Yellow Dwarf Viruses

Author
item DEB, MAHUA - PURDUE UNIV.
item ANDERSON, JOSEPH

Submitted to: American Oat Workers Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/24/2006
Publication Date: 10/13/2006
Citation: Deb, M., Anderson, J.M. 2006. Development of a rapid detection method for Yellow Dwarf Viruses. American Oat Workers Conference Proceedings. p. 64.

Interpretive Summary:

Technical Abstract: Barley and Cereal yellow dwarf viruses (B/CYDVs), constitute the most economically important group of oat viruses. A multiplex reverse transcription polymerase chain reaction method was developed for the simultaneous detection and discrimination of five B/CYDVs viruses. The protocol uses specific primers sets for each virus producing five distinct fragments 294 bp, 179 bp, 238 bp, 397 bp and 331 bp, indicating the presence of all three strains of BYDVs, -PAV, -MAV, -SGV and two strains of CYDVs, -RPV and -RMV respectively. The amplification specificity of these primers was tested against wide range of field samples from different parts of United States. Another version of the protocol utilizes fluorescently tagged primers which streamlines the detection of each virus through fluorescent capillary electrophoresis. This method fulfills the need for a fast and specific method to detect any of these five viruses. This method will be an important diagnostic tool and will also be very useful in examining the epidemiology of these viral diseases.