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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #204977
Title: The DcMaster Transposon Display maps polymorphic insertion sites in the carrot (Daucus carota L.) genome

Author
item GRZEBELUS, DARIUSZ - UNIV OF KRAKOW, POLAND
item JAGOSZ, BARBARA - UNIV OF KRAKOW, POLAND
item Simon, Philipp

Submitted to: Gene
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/20/2006
Publication Date: 1/1/2007
Citation: Grzebelus, D., Jagosz, B., Simon, P.W. 2007. The DcMaster Transposon Display maps polymorphic insertion sites in the carrot (Daucus carota L.) genome. Gene. 390:67-74.

Interpretive Summary: While most of the DNA in chromosomes is very stable, some pieces of DNA, referred to as transposable elements, do more to various locations as chromosomes. We discovered and described a new transposable element in carrots recently and in this study we found this element, or parts of it, are located at 77 places along carrot chromosomes. The relative location of these elements was random. Prospects for their future movement was considered to be quite likely. This information is of interest to geneticists, molecular biologists, and carrot breeders.

Technical Abstract: DcMaster is a family of PIF/Harbinger-like class II transposable elements identified in carrot. We present a modified Transposon Display molecular marker system allowing amplification of genomic regions containing DcMaster elements. We scored 77 DcMaster Transposon Display (DcMTD) amplicons, of which 54 (70%) were segregating in the F2 progeny from the cross between wild and cultivated carrot. Segregating amplicons were incorporated into a previously developed molecular linkage map of carrot. Twenty-eight markers were attributed to the wild parent, 23 originated from the cultivated parent, and three markers remained unlinked. The markers were evenly distributed among the nine linkage groups. However, differences in the distribution pattern of DcMaster insertion sites in the genomes of the wild and cultivated parent were observed. Specificity of the obtained amplicons was confirmed by sequencing and three putative DcMaster subfamilies, differing in the sequence of their terminal inverted repeats, were revealed.