Author
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ROBERTSON, NANCY |
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Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 5/4/2007 Publication Date: 9/20/2007 Citation: Robertson, N.L. 2007. Identification and characterization of a new virus in the genus Potyvirus from wild populations of Angelica lucida L. and A. genuflexa Nutt., family Apiacea. Archives of Virology. 152(9):1603-1611. DOI 10.1007/s00705-007-1003-4. Interpretive Summary: Native plants in the Matanuska-Susitna Valley grow either in natural ecosystems or in remnant lands interspersed in residential or farm lands. In 2004, diseased wild celery, Angelica lucida L., was diagnosed with an unknown plant virus near crops, wooded lots, and residential houses. Subsequently, in 2005-06, surveys for diseased wild celery plants were conducted on the original site and in a remote natural ecosystem. The objective was to identify the virus and study its biology. Wild celery and Angelica genuflexa Nutt. (kneeling angelica) were the only two naturally infected host, with the later confined to the natural ecosystem. Experimental plant host for the virus included carrots, parsley, celery, and dills. Preliminary surveys in the carrot crops were negative for the virus. The virus morphology and partial genomic sequence gave definitive identification as a new species, tentatively named Angelica virus Y (AnVY), in the genus Potyvirus, family Potyviridae. It is not clear if the virus originated in a native plant species or from domestic plant species. The results of this survey are important to the vegetable farmers and natural resource managers in south central Alaska in that a potential exists for AnVY to spread into selected crops and other native plants. Technical Abstract: A novel potyvirus was discovered in Angelica livida L. (wild celery) and Angelica genuflexa Nutt. (kneeling Angelica) (family Apiaceae) in the Matanuska-Susitna Valley, Alaska. The experimental plant host range of the virus included species in three families: Chenopodiaceae (Chenopodium amaranticolor Coste et Reyn and Chenopodium quinoa Willd., Solanaceae (Nicotiana benthamiana Domin, and N. clevlandii Gray., and Apiaceae (Anethum graveolens L., Apium graveolens L. var. dulce (Miller)), Daucus carota L. subspecies sativus (Hoffm.) Arcang., and Petroselinum crispum (Miller) Nyman ex A. W. Hilland. The virus contained flexuous rods with an ssRNA genome ~9.2-9.5 and a CP (~35 kDa) that reacted to a universal potyvirus monoclonal antibody in western analysis. The sequenced genomic 3'-end (~1,850 nt) contained a potyvirus genomic arrangement that included the 3'-terminus of the NIb (nuclear inclusion) gene, the CP (coat protein) gene, and a 3’-UTR (untranslated region) attached to a poly(A)tail. The CP amino acids had between 54-70 % identities with 12 selected members from the genus Potyvirus. Phylogenetically, the Alaskan potyvirus clustered with three other apiaceous potyvirus virus from Australia. The novel Alaskan virus, confined naturally to A. livida L. and A. genuflexa Nutt., was classified in the genus Potyvirus, family Potyviridae, and in part named after its natural plant host, angelica virus Y (AnVY). |
