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Title: Diversity of tri-functional histidine biosynthesis gene (his) in cereal Phaeosphaeria species

Author
item WANG, CHIH-LI - KAOHSIUNG TAIWAN
item MALKUS, ARKADIUSZ - RADZIKOW POLAND
item ZUZGA, SABINA - WARSAW POLAND
item CHANG, PI-FANG - TAICHUNG TAIWAN
item ARSENIUK, EDWARD - RADZIKOW POLAND
item Ueng, Peter

Submitted to: Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/26/2007
Publication Date: 7/6/2007
Citation: Wang, C., Malkus, A., Zuzga, S.M., Chang, P.L., Arseniuk, E., Ueng, P.P. 2007. Diversity of tri-functional histidine biosynthesis gene (his) in cereal Phaeosphaeria species. Genome. 50:595-609.

Interpretive Summary: Septoria disease is responsible for significant economic losses in the production of cereal crops. The disease is caused by a complex of several different species of fungus that are difficult to distinguish from one another, hindering efforts to devise measures for fungus-specific disease management. From the Septoria disease fungi, we isolated and characterized the gene that is involved in amino acid (histidine) biosynthesis. Results from this study showed that this gene and the protein that it codes for, provide useful molecular markers to aid detection and differentiation of the septoria disease fungi. The findings will be of interest to scientists and diagnostics laboratories.

Technical Abstract: The full length genomic sequences of tri-functional histidine biosynthesis (his) gene were obtained and compared from cereal Phaeosphaeria species by PCR amplification. The his gene coding sequence in wheat-biotype P. nodorum (PN-w) was 2697 bp in size. The his genes in barley-biotype P. nodorum (PN-b), two P. avenaria f.sp. triticea including homothallic Pat1 and Pat3, and Phaeosphaeria spp. from Polish ryes and dallis grass were 2694 bp which were 3 bp shorter than PN-w. The his gene in heterothallic Pat2 was 2693 bp, due to one base less in the intron. In P. avenaria f.sp. avenaria (Paa), the his gene was shorter, only 2670 bp in length. The difference in the his gene size contributed to size variation of amino acid sequences in the gap region located between phosphoribosyl-ATP pyrophosphohydrolase (PRA-PH) and histidinol dehydrogenase (HDH) sub domains. Based on nucleotide and deduced amino acid sequences of the his gene, homothallic Pat1 was not closely related to neither PN-w nor Paa clade. It appeared that evolution rates of the his gene were fast in cereal Phaeosphaeria species.