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Title: Screening of F1/F2 seed for the presence of a molecular marker for Sclerotinia minor resistance

Author
item Chamberlin, Kelly
item DAMICONE, JOHN - OSU DEPT OF ENTOMOLOGY

Submitted to: Peanut Research at Oklahoma State University
Publication Type: Experiment Station
Publication Acceptance Date: 11/15/2006
Publication Date: 12/1/2006
Citation: Chenault, K.D., Damicone, J. 2006. Screening of F1/F2 seed for the presence of a molecular marker for Sclerotinia minor resistance. In: Partners and Progress - Peanut Research at OSU, 2005. Oklahoma Agricultural Experiment Station, P-1013.

Interpretive Summary:

Technical Abstract: Fungal diseases of peanut, such as Sclerotinia blight caused by Sclerotinia minor, are responsible for increased production costs and incredible yield losses for peanut producers in Oklahoma. Current Sclerotinia blight management strategies include cultivar selection and expensive fungicide application. Recently, the Southwestern Sheller Industry has abruptly discontinued seed production of cultivars that exhibit the hard roasted kernel trait, including the most widely grown disease resistant and/or high oleic varieties Tamrun 96 and Tamrun OL-01. The same industry has also begun to require that only peanut seed that have high oleic acid content will be contracted. Oklahoma producers are currently without adequate replacement cultivars. This project is part of a research program that will produce new cultivars suitable for commercial production in Oklahoma. These new cultivars will have improved resistance to fungal pathogens, be high oleic in nature, and will not have the hard-roasted kernel trait. Crosses have already been made using parents that have elevated resistance to Sclerotinia minor (S. minor) and parents that have high oleic acid content. Also, a genetic marker for S. minor resistance has been identified and a technique has been developed by which DNA can be extracted from a small portion of the peanut seed while leaving the seed viable. By screening peanut seed for the presence of the marker for S. minor resistance, potentially resistant seed can be identified at the F1/F2 generation and the efficiency of developing new peanut cultivars will be greatly enhanced. Genetic screening for the resistance marker is just one part of a three-component approach to early generation analysis, the other two parts being near-infrared reflectance (NIR) analysis and greenhouse testing for S. minor resistance. The objective of this project was to screen F1 and F2 seed for the presence of the Sclerotinia minor resistance marker and for high oleic acid content.