Author
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CREGAN, PERRY |
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Submitted to: Genetics and Genomics of Soybean
Publication Type: Book / Chapter Publication Acceptance Date: 4/15/2008 Publication Date: 5/25/2008 Citation: Cregan, P.B. 2008. The Soybean Molecular Genetic Linkage Map. Genetics and Genomics of Soybean. 79-89. Interpretive Summary: Technical Abstract: A brief history of molecular genetic mapping in soybean [Glycine max (L.) Merr.] is followed by a description of the most current version of the soybean molecular genetic linkage map which is primarily based simple sequence repeat (SSR) or microsatellite markers as well as single nucleotide polymorphism (SNP)-based markers. Like many plant and animal species, the first molecular map of soybean was based on restriction fragment length polymorphism (RFLP) markers. Because of the relatively low level of sequence diversity in soybean, the first RFLP maps were constructed in populations derived from crosses of cultivated (G. max) x wild soybean [G. soja (Seib. et Zucc.)]. Random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) loci were briefly used in map construction, but soybean was the first plant species in which simple sequence repeat (SSR) markers were employed and SSRs soon became the marker of choice in map development. Single nucleotide polymorphism (SNP) markers are now being widely used by plant and animal geneticists and the most recent molecular genetic map of soybean incorporates SNPs from more than 1100 different genes. This map provides an initial iteration of a soybean transcript map and more than doubles the number of mapped PCR-based markers available to soybean geneticists and breeders. |
