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ARS Home » Southeast Area » Raleigh, North Carolina » Soybean and Nitrogen Fixation Research » Research » Publications at this Location » Publication #210328
Title: Molecular Mapping and Confirmation of QTL Associated with Oleic Acid Content in N00-3350 Soybean

Author
item MONTEROS, MARIA - UNIV OF GEORGIA
item BURTON, JOSEPH
item BOERMA, H. - UNIV OF GEORGIA

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/26/2008
Publication Date: 11/24/2008
Citation: Monteros, M.J., Burton, J.W., Boerma, H.R. 2008. Molecular Mapping and Confirmation of QTL Associated with Oleic Acid Content in N00-3350 Soybean. Crop Sci. 48:2223-2234.

Interpretive Summary: The fatty acid composition of soybean oil affects the flavor, nutritional value, and stability when used for frying. If the oleic fatty acid is increased in soybean oil, stability is increased and the need to hydrogenate the oil is reduced. Hydrogenation of vegetable oil creates unhealthy trans fatty acids. Soybeans with high oleic acid in seed oil have been developed through standard plant breeding practice. It was determined that six genes control the high oleic trait in these soybeans. DNA markers were identified that are associated with these genes and their location in the soybean genome was determined. This information will be extremely useful for soybean breeders who are developing soybean varieties with increased oleic acid.

Technical Abstract: The fatty acid composition of soybean [Glycine max (L.) Merr.] seed affects the flavor, nutritional value, and stability of the oil. Oleic acid is one of the major fatty acids in soybean, with most current cultivars possessing 160 to 280 g kg-1. Increasing oleic acid content in soybean oil would reduce the need for hydrogenation, a process that creates unhealthy trans fatty acids. The objective of this study was to map and confirm the areas of the soybean genome associated with oleic acid content from N00-3350 (~583 g kg-1oleic acid) using simple sequence repeat (SSR) markers. A F2:3 population of 259 lines from the cross of G99-G725 ' N00-3350 was used as a mapping population, and a F2:3 population of 231 lines from the cross of G99-G3438 ' N00-3350 was used as a confirmation population. Based on single factor analysis of variance (ANOVA), interval mapping and composite interval mapping (CIM), six QTLs for oleic acid content were found on linkage groups (LG) A1 (Satt211, R2 = 4%), LG-D2 (Satt389, R2= 6%), LG-G (Satt394, R2=13%), LG-G (Satt191, R2=7%), LG-L (Satt418, R2=9%), and LG-L (Satt561, R2=25%) in the G99-G725 ' N00-3350 population. All six QTLs for oleic acid were confirmed in the G99-3438 ' N00-3350 population. At all of the identified oleic acid QTLs, the N00-3350 allele increased the oleic acid content. We propose the designation cqOle2-1, cqOle2-2, cqOle2-3, cqOle2-4, cqOle2-5, and cqOle2-6 for the oleic acid QTL that have been identified and confirmed. The identification of SSR markers closely linked to the oleic acid QTLs will facilitate the use of marker-assisted selection (MAS) in soybean breeding programs to increase the oleic acid content in soybean seed.