Author
 |
Scupham, Alexandra |
|
Wesley, Irene |
|
Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 4/19/2007 Publication Date: 4/19/2007 Citation: Scupham, A.J., Wesley, I.V. 2007. Campylobacter community development in the context of significant gut microbiota transition [abstract]. Institute for Food Safety and Security. p. 13. Interpretive Summary: Technical Abstract: Campylobacter colonization of poultry causes a considerable public health risk. Interactions between the pathogen and the autochthonous intestinal microbiota have not been defined, however, Campylobacter can be excluded from the intestinal habitat by unidentified microbial species. To enhance our understanding of intestinal microbial dynamics, community composition in the ceca of turkeys was examined. Weekly sampling was performed for two flocks throughout the brooding and grow-out phases at a standard two-stage commercial turkey production facility, with five birds samples at every time point. The two flocks were housed roughly three miles apart, and the flocks were placed in the brooders within two weeks of one another so sampling occurred roughly concurrently. Cecal contents were frozen for DNA analysis and subsamples were cultured for Campylobacter using the South African filter method. Community fingerprint analysis of isolated DNA by Automated Ribosomal Intergenic Spacer Analysis (ARISA) confirmed a previous observation that turkey cecal communities undergo a significant compositional change around week 11. The transition includes increases in bacteroides populations and decreases in Clostridia populations. Campylobacter isolates cultured during the period of community change were identified by Amplified Fragment Length Polymorphism (AFLP) to include both C. jejuni and C. coli. |
