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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #212467
Title: Detection of respiratory viruses and subtype identification of influenza viruses by GreeneChipResp oligonucleotide microarray

Author
item QUAN, P - COLUMBIA UNIVERSITY
item PALACIOS, G - COLUMBIA UNIVERSITY
item JABADO, O - COLUMBIA UNIVERSITY
item CONLAN, S - COLUMBIA UNIVERSITY
item HIRSCHBERG, D - STANFORD SCHOOL OF MED
item POZO, F - CENTRO NACIONAL DE MICROB
item JACK, P - AUSTRALIAN ANIMAL HEALTH
item CISTERNA, D - INSTITUTO NACIONAL DE ENF
item RENWICK, N - COLUMBIA UNIVERSITY
item HUI, J - COLUMBIA UNIVERSITY
item DRYSDALE A, A - COLUMBIA UNIVERSITY
item BAUMEISTER, E - INSTITUTO NACIONAL DE ENF
item SAVY, V - INSTITUTO NACIONAL DE ENF
item Lager, Kelly
item Richt, Juergen
item BOYLE, D - AUSTRALIAN ANIMAL HEALTH
item WEBBY, R - ST JUDE CHILDREN'S RESEAR
item GARCIA-SASTRE, A - MOUNT SINAI SCHOOL OF MED
item CASAS, I - CENTRO NACIONAL DE MICROB
item PEREX-BRENA, P - CENTRO NACIONAL DE MICROB
item BRIESE, T - COLUMBIA UNIVERSITY
item LIPKIN, W - COLUMBIA UNIVERSITY

Submitted to: Journal of Clinical Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/21/2007
Publication Date: 8/1/2007
Citation: Quan, P.L., Palacios, G., Jabado, O.J., Conlan, S., Hirschberg, D.L., Pozo, F., Jack, P.J., Cisterna, D., Renwick, N., Hui, J., Drysdale A., Amos-Ritchie, R., Baumeister, E., Savy, V., Lager, K.M., Richt, J.A., Boyle, D.B., Garcia-Sastre, A., Casas, I., Perez-Brena, P., Briese, T., Lipkin, W.I. 2007. Detection of respiratory viruses and subtype identification of influenza A viruses by GreeneChipResp oligonucleotide microarray. Journal of Clinical Microbiology. 45(8):2359-2364.

Interpretive Summary: Influenza A virus infections are a significant causes of morbidity and economic burden for the pig industry. We have developed a fast and reliable detection system for influenza A viruses of pigs. All influenza viruses circulating in U.S. pigs can be easily identified using this detection system. In addition, this assay allows accurate identification of 21 different respiratory virus species from animals and humans. This information will be useful to livestock health officials and USDA action agencies such as USDA/APHIS.

Technical Abstract: Acute respiratory infections are a significant cause of morbidity, mortality and economic burden worldwide. Accurate, early differential diagnosis may alter individual clinical management as well as facilitate recognition of outbreaks that have implications for public health. Here we report the establishment and validation of a comprehensive and sensitive microarray system for detection and speciation of respiratory viruses in clinical materials. Implementation of a set of influenza enrichment primers facilitated subtyping of influenza A viruses through differential recognition of hemagglutinins 1 through 16 and neuraminidases 1 through 9. Twenty-one different respiratory virus species were accurately characterized including a recently identified novel genetic clade of rhinovirus.