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Title: A metagenomic survey of microbes in honey bee colony collapse disorder

Author
item COX-FOSTER, DIANA - PENNSYLVANIA STATE UNIV
item CONLAN, SEAN - COLUMBIA UNIV
item HOLMES, EDWARD - PENNSYLVANIA STATE UNIV
item PALACIOS, GUSTAVO - COLUMBIA UNIV
item Evans, Jay
item MORAN, NANCY - UNIV OF ARIZONA
item QUAN, PHENIX-LAN - COLUMBIA UNIV
item BRIESE, THOMAS - COLUMBIA UNIV
item HORNIG, MADY - COLUMBIA UNIV
item GEISER, DAVID - PENNSYLVANIA STATE UNIV
item MARTINSON, VINCE - UNIV OF ARIZONA
item VANENGELSDORP, DENNIS - PA DEPT OF AGRICULTURE
item KALKSTEIN, ABBY - PENNSYLVANIA STATE UNIV
item DRYSDALE, ANDREW - COLUMBIA UNIV
item HUI, JEFFREY - COLUMBIA UNIV
item ZHAI, JUNHUI - COLUMBIA UNIV
item CUI, LIWANG - PENNSYLVANIA STATE UNIV
item HUTCHISON, STEPHEN - 454 LIFE SCIENCES
item SIMONS, JAN FREDRIK - 454 LIFE SCIENCES
item EGHOLM, MICHAEL - 454 LIFE SCIENCES
item Pettis, Jeffery
item LIPKIN, W. IAN - COLUMBIA UNIV

Submitted to: Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/30/2007
Publication Date: 9/6/2007
Citation: Cox-Foster, D.L., Conlan, S., Holmes, E.C., Palacios, G., Evans, J.D., Moran, N.A., Quan, P., Briese, T., Hornig, M., Geiser, D.M., Martinson, V., Vanengelsdorp, D., Kalkstein, A.L., Drysdale, A., Hui, J., Zhai, J., Cui, L., Hutchison, S.K., Simons, J., Egholm, M., Pettis, J.S., Lipkin, W. 2007. A metagenomic survey of microbes in honey bee colony collapse disorder. Science. 318:283-287.

Interpretive Summary: In Colony Collapse Disorder (CCD), honey bee colonies inexplicably lose all of their workers. CCD has resulted in a loss of 50-90% of colonies in beekeeping operations across the United States. The observation that irradiated combs from affected colonies can be repopulated with naïve bees suggests an infectious basis for CCD. We used an unbiased metagenomic approach to survey the bacteria, fungi and viruses associated with CCD and normal hives. Candidate pathogens were screened for significance of association with CCD by examination of samples collected from several sites over a period of three years. One organism, Israeli acute paralysis virus (IAPV) of bees, was strongly correlated with CCD and also found in bees recently imported from Australia.

Technical Abstract: In Colony Collapse Disorder (CCD), honey bee colonies inexplicably lose all of their workers. CCD has resulted in a loss of 50-90% of colonies in beekeeping operations across the United States. The observation that irradiated combs from affected colonies can be repopulated with naïve bees suggests an infectious basis for CCD. We used an unbiased metagenomic approach to survey microflora in CCD hives, normal hives and imported royal jelly. Candidate pathogens were screened for significance of association with CCD by examination of samples collected from several sites over a period of three years. One organism, Israeli acute paralysis virus (IAPV) of bees, was strongly correlated with CCD. We have not proven a causal relationship between any infectious agent and CCD; nonetheless, the prevalence of IAPV sequences in CCD operations, as well as the temporal and geographic overlap of CCD and importation of IAPV infected bees from Australia, indicates that IAPV is a significant marker for CCD.