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Title: Microsatellite Diversity of Soybean Genotypes Differing in Bean Pod Mottle Virus Leaf Symptom

Author
item Mian, Rouf
item KANG, S - SUWON, KOREA
item Redinbaugh, Margaret

Submitted to: Canadian Journal of Plant Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/30/2008
Publication Date: 3/1/2009
Citation: Mian, R.M., Kang, S., Redinbaugh, M.G. 2009. Microsatellite Diversity of Soybean Genotypes Differing in Bean Pod Mottle Virus Leaf Symptom. Genetic Resources and Crop Evolution. 89:359-67.

Interpretive Summary: Soybean is the most important source of vegetable oil and protein meal in the world. Bean pod mottle virus (BPMV) can reduce soybean yield and productivity in most soybean growing states of the USA. Partial resistance or field resistance of soybean to the virus can minimize the yield loss. Through a series of greenhouse screenings we have identified several plant introductions with large significant (P'0.001) differences in expression of BPMV leaf symptoms. A microsatellite diversity study on 48 soybean genotypes differing in leaf symptoms of BPMV using 271 markers covering all 20 linkage groups of the composite soybean genetic map was conducted. The soybean genotypes clustered into distinct groups based on their country of origin and/or their BPMV leaf symptoms. Based on the microsatellite data, the genotypes were clustered into seven groups. Groups 1, 2 and 4 all included genotypes from China only, group 3 included all genotypes from the USA, and group 7 included genotypes from Japan only. Group 1 consisted of genotypes with low leaf symptoms of BPMV while group 6 was formed with genotypes with high leaf symptoms of BPMV. The results of this study will facilitate selection of highly polymorphic parental lines for developing mapping populations that will segregate for bean pod mottle virus leaf symptoms.

Technical Abstract: Soybean, Glycine max (L.) Merr, is the most important source of vegetable oil and protein meal in the world. Bean pod mottle virus (BPMV) is a threat to soybean yield and productivity in most soybean growing states of the USA. In the absence of complete resistance to BPMV, partial resistance of soybean to this virus can play an important role in combating BPMV. The objectives of this study were to: (i) evaluate 600 plant introductions (PIs) for expression of leaf symptoms under greenhouse conditions following inoculation with an Ohio isolate of BMPV, and (ii) determine the genetic diversity among 42 soybean PIs with differences in leaf symptoms of BPMV and six high yielding soybean lines adapted to Ohio. Large significant (P'0.001) differences for expression of BPMV leaf symptoms in the greenhouse were detected. A genetic diversity study on 48 soybean genotypes differing in leaf symptoms of BPMV using 271 microsatellite markers covering all 20 linkage groups of the composite soybean genetic map was conducted. The average polymorphism information content for the microsatellites was 0.53 and the average number of loci per microsatellite was 3.23. The soybean genotypes clustered into distinct groups based on their country of origin and/or their BPMV leaf symptoms. Based on the microsatellite data, the genotypes were clustered into seven groups. Groups 1, 2 and 4 all included genotypes from China only, group 3 included all genotypes from the USA, and group 7 included genotypes from Japan only. Group 1 consisted of genotypes with low leaf symptoms of BPMV while group 6 was formed with genotypes with high leaf symptoms of BPMV.