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Submitted to: Entomological Research (Korea)
Publication Type: Abstract Only Publication Acceptance Date: 7/26/2007 Publication Date: 8/23/2007 Citation: Functional genes of non-model arthropods, Margaret Louise Allen, Entomological Research, Volume 37, Supplement 1, August 2007 page A77 (International Congress of Insect Biotechnology and Industry Proceedings, 19-24 August 2007, Daegu, Republic of Korea) ISSN 1738-2297 (Print) ISSN 1748-5967 (online) Published by Blackwell Interpretive Summary: Technical Abstract: Technology and bioinformatics facilitate studies of non-model organisms, including pest insects and insect biological control agents. A cDNA library prepared from a laboratory-reared colony of Lygus lineolaris male nymphs identified sequences that appeared to have known functions or close homologues and have been targeted for further study. Sequences of interest include actins, tubulins, ribosomal subunits, proteinases, polygalacturonases (PGs) and cuticle genes. Expression profiles for some of these putative genes have been obtained. Total RNA samples were collected from eggs at 3 and 8 days of development, 2nd instar nymphs, 5th instar nymphs (identifiable as either male or female) and adults, male and female. Equal quantities of total RNA were reverse transcribed and used for semiquantitative PCR. Several genes, including the PGs, were expressed with stage- and sex-specificity. Constitutively expressed genes were identified and will be useful as standards. Genes that may be used as promoters in transgene vectors, and those that may be tested in double stranded RNA gene knockdown experiments have been identified. |
