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Title: Characterization and phylogenic analysis of Mexican Newcastle disease virus isolates

Author
item MERINO, R - UNAM, MEXICO
item CALDERON, N - UNAM, MEXICO
item PEROZO, F - UNIV GEORGIA, PDRC
item VILLEGAS, P - UNIV GEORGIA, PRDC
item Afonso, Claudio

Submitted to: International Poultry Forum Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/7/2007
Publication Date: 1/21/2008
Citation: Merino, R., Calderon, N., Perozo, F., Villegas, P., Afonso, C.L. 2008. Characterization and phylogenic analysis of Mexican Newcastle disease virus isolates [abstract]. International Poultry Scientific Forum, January 21-22, 2008, Atlanta, Georgia. p. 33.

Interpretive Summary:

Technical Abstract: Newcastle disease virus (NDV) was isolated in Mexico for the first time in 1946 and the last report of a field outbreak caused by a highly virulent strain dates from year 2000, when 13.6 million birds were slaughtered and 93 farms quarantined. Mean Death Time test resulted in velogenic classification of 12 Mexican NDV strains, isolated around Mexico City between 1946 and 2006. Analysis of the deduced amino acid sequences of the F protein cleavage site from these isolates predicted that 11 were velogenc viruses, while one isolate was classified as Lentogen (like Ulster). A phylogenetic tree based on the nucleotide sequences of the F gene revealed that these Mexican NDV isolates corresponded to gnotypes I, V and VIII (one, 10 and one isolates, respectively). Isolates belonging to genotype V are related to isolate Largo71 (isolates from 1946, 2004, 2005 and 2006) and GamefowlUS/02 (isolates from 1998, 2000 and 2001). The virus classified into genotype VIII was isolated around 1947 and this is the first time that is analyzed by molecular biology, however, it has been used in Mexico as challenge strain. The distinctive genomic differences between the two groups of genotype V viruses (isolates from 1998 to 2001 and from 2004 to 2006) suggest that two different lineages of NDV are challenging the poultry industry in the central region of Mexico.