Author
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ZINGG, JEAN-MARC - JM USDA HNRCA @ TUFTS |
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NEGIS, YESIM - JM USDA HNRCA @ TUFTS |
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GIANELLO, ROBERT - MONASH UNIV AUSTRALIA |
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LIBINAKI, ROKSAN - MONASH UNIV AUSTRALIA |
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OGRU, ESRA - MONASH UNIV AUSTRALIA |
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Meydani, Mohsen |
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AZZI, ANGELO - JM USDA HNRCA @ TUFTS |
Submitted to: Experimental Biology
Publication Type: Abstract Only Publication Acceptance Date: 12/1/2007 Publication Date: 4/5/2008 Citation: Zingg, J., Negis, Y., Gianello, R., Libinaki, R., Ogru, E., Meydani, M., Azzi, A. 2008. Molecular and cellular effects of a-, and g-tocopheryl phosphate on THP-1 monocytes. Experimental Biology Annual Meeting. Abstract No. 1103.7. Interpretive Summary: Technical Abstract: The vitamin E derivative, alpha-tocopheryl phosphate (aTP), can be isolated from food and animal tissues in amounts of nmol/g of extracted material. Whereas the phosphate ester increases the chemical stability of alpha-tocopherol (aT), it suppresses its function as a direct free radical scavenger. Nevertheless, in cells aTP influences signal transduction and gene expression, possibly by interacting with specific enzyme(s) and/or receptor(s). Small amounts of aTP formed from aT in cultured cells further suggest a physiological role for this compound. We have now compared the inhibitory effects of aTP (acid and sodium salt) and g-tocopheryl phosphate (gTP) (acid and sodium salt) on the proliferation of THP-1 monocytic leukemia cells. aT does not affect the cell proliferation rate in these cells, whereas aTP and more potently gTP inhibit THP-1 cell proliferation. We found that one or more cell anion transporters are modulating the response of THP-1 cells to the treatment with aTP and gTP, as determined by using specific transport inhibitors, such as glybenclamide/glyburide. In addition, the effects of these vitamin E derivatives on gene expression are assessed, in particular on the inhibition of CD36 scavenger receptor gene expression. |