Author
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ANDERSON, JOSEPH |
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Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only Publication Acceptance Date: 12/1/2007 Publication Date: 1/12/2008 Citation: Anderson, J.M. 2008. Overview of Oat Microsatellite Development [abstract]. Plant and Animal Genome XVI Conference. Paper No. W328. Interpretive Summary: Technical Abstract: Oat genetics and breeding is handicapped because of the dearth of molecular markers. Development of elite germplasm and varieties has thus far relied on DNA markers primarily developed in other grasses such as wheat and rice. However, a low percentage of wheat and rice PCR-based markers transfer to oat and provide useful polymorphic data. One type of useful PCR-based marker are DNA sequences which contain Simple Sequence Repeats (SSRs) and this type of sequence has been every useful as markers in other plant and animal systems. To date, there are approximately 150 SSR markers in oat which are insufficient to provide a reasonable amount of genome coverage and to facility marker-assisted selection in a breeding program. In an effort to rectify this situation my laboratory has developed SSR-enriched libraries for 8 different di- and tri-nucleotide repeats from Kanota and Ogle DNA digested with four different restriction enzymes to ensure coverage across the genome. We have sequenced 13,824 clones from these 16 libraries and have identified nearly 1500 sequences that contain SSRs. We have prepared primer pairs to 768 different genomic SSR containing sequences and have tested 384 for amplicon(s) production and if the amplicons are polymorphic between Kanota and Ogle and two Purdue parental lines on high resolution agarose gels. Our current efforts are geared towards mapping the polymorphic SSRs on the Kanota/Ogle mapping population. These results and our collaborations that have greatly enhanced this project will be discussed. |
