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Title: LC-MS analysis of solanidane glycoalkaloid diversity among four wild potato species and three cultivars

Author
item SHAKYA, ROSHANI - WASHINGTON ST UNIV
item Navarre, Duroy - Roy

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/1/2008
Publication Date: 7/11/2008
Citation: Shakya, R., Navarre, D.A. 2008. Lc-ms analysis of solanidane glycoalkaloid diversity among four wild potato species and three cultivars. Journal of Agricultural and Food Chemistry. DOI 10.1021/jf8006618. 56:6949-58.

Interpretive Summary: Potatoes have compounds called glycoalkaloids that are involved in resistance to pests and pathogens. These glycoalkaloids also have both positive and negative health effects. We used LCMS analysis to identify over 45 solanidane GAs in potato tubers, of which several have not been previously reported. Four wild species and three cultivars were examined. The total number of GAs found suggests potatoes have more types of GAs than previously expected. The ability to monitor so many GAs creates new opportunities to assess the role of specific GAs in plant and pest resistance. Some GAs are known to be substantially less toxic than others and some have anti-cancer properties. This method will assist in determining which GAs have health-promoting potential.

Technical Abstract: Among the small molecules found in potato tubers are some that are phytonutrients or plant defense compounds. The extent of variation of these small molecules among different potato genotypes is not well characterized. LCMS analysis of tuber extracts from seven potato genotypes showed that one large source of small molecule variation among these genotypes were the glycoalkaloids. Glycoalkaloids (GAs) are involved in the resistance of potatoes to pathogens and pests, but they also have implications for human health and nutrition. This study focused on GAs with solanidane derived aglycones, of which over 45 were identified and included several novel GAs. This analysis suggested the variety of GAs in tubers is considerably greater than previously realized.