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ARS Home » Pacific West Area » Corvallis, Oregon » National Clonal Germplasm Repository » Research » Publications at this Location » Publication #222022
Title: Polyvinyl Pyrrolidone is not Effective for Improving Recovery of Cryopreserved Shoots of Two Rubus Cultivars

Author
item MUMINOVA, MAGFRAT - TASHKENT UZBBEKISTAN
item Reed, Barbara

Submitted to: In Vitro Cellular and Developmental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 4/16/2008
Publication Date: 6/13/2008
Citation: Muminova, M., Reed, B.M. 2008. Polyvinyl Pyrrolidone is not Effective for Improving Recovery of Cryopreserved Shoots of Two Rubus Cultivars. In Vitro Cellular and Developmental Biology. 44:2022.

Interpretive Summary: At the USDA ARS genebank in Corvallis, Oregon, we are interested in the long-term preservation of blackberry plants. We are looking for ways to improve storage in liquid nitrogen (cryopreservation). Storing plant cells in liquid nitrogen imposes stresses on plant cells and tissues that result in increased oxidation and damage. Antioxidant chemicals may be useful in decreasing this damage and improving recovery after cryopreservation. The antioxidant chemical polyvinyl pyrrolidone (PVP) was tested as a cryopreservation pretreatment or recovery treatment for blackberry shoot cultures. Plantlet growth and multiplication during pretreatment was severely inhibited at all but the lowest concentration in the medium. Following cryopreservation, regrowth of plantlets pretreated with 1% or 2.5 % PVP was similar to the controls; however, plantlets pretreated with 5% or 10% PVP had much less regrowth. PVP in the recovery medium produced less regrowth than the control medium in all cases and reduced recovery of plantlets after cryopreservation from 60% to less than 20%. These results indicate that PVP has no effect or is detrimental to the successful cryopreservation and subsequent recovery of blackberry shoot tips.

Technical Abstract: The use of the antioxidant polyvinyl pyrrolidone as a pretreatment or recovery treatment for Rubus shoot cultures was tested with two blackberry cultivars. Plantlet growth was initially evaluated on medium with 1 to 10% PVP (10,000 MW). Growth and multiplication of the plantlets was severely inhibited with 2.5% to 10% PVP in the medium. Following cryopreservation, recovery of plantlets pretreated for 48 h on 5% DMSO medium with 1% or 2.5 % PVP was not significantly different from the controls; however, plantlets pretreated with 5% or 10% PVP had significantly less regrowth. PVP in the recovery medium produced significantly lower regrowth than the control medium in all cases and reduced recovery of plantlets after cryopreservation from 60% to less than 20%. These results indicate that PVP has no effect or is detrimental to the successful cryopreservation and subsequent recovery of Rubus shoot tips.