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Title: Expressed cDNAs from embryonic and larval stages of the horn fly (Diptera: Muscidae)

Author
item Guerrero, Felicito
item Dowd, Scot
item NENE, V - U OF MARYLAND, BALTIMORE
item FOIL, L - LSU BATON ROUGE LA

Submitted to: Journal of Medical Entomology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/7/2008
Publication Date: 7/1/2008
Citation: Guerrero, F., Dowd, S.E., Nene, V.M., Foil, L.D. 2008. Expressed cDNAs from embryonic and larval stages of the horn fly (Diptera: Muscidae). Journal of Medical Entomology. 45(4):686-692

Interpretive Summary: We synthesized two cDNA libraries from RNA isolated from embryos and 1st instar larvae from a Louisiana field population of horn flies. DNA sequence information from approximately 10,000 library clones was obtained and assembled into embryo and larval horn fly DNA sequence databases. Additionally, sequence data from both libraries was pooled to produce a database containing over 4,300 sequences from both life stages of the fly. Gene function was assigned when available matches to genes with known function were available. A number of sequences were identified which may have roles in the horn fly's increasing resistance to insecticides.

Technical Abstract: We used an expressed sequence tag approach to initiate a study of the genome of the horn fly, Haematobia irritans. Two normalized cDNA libraries were synthesized from RNA isolated from embryos and 1st instar larvae from a field population of horn flies. Approximately 10,000 clones were sequenced from both the 5' and 3' directions. Sequence data from each library was assembled into a database of tentative consensus sequences (TCs) and singletons and used to search public protein databases. Additionally, the data from both the egg and larval ESTs were combined into a single database consisting of 16,702 ESTs assembling into 2886 TCs and 1522 singleton entries. A number of sequences were identified which may have roles in the horn fly's increasing resistance to insecticides.